C57Bl/6 mice received weekly i.v. injections with 300 ng PTx in 200 µl of PBS or PBS alone. S. WeberMartin BenkhouchaMahdia Lehmann-HornKlaus HertzenbergDeetje SellnerJohann Santiago-RaberMarie-Laure ChofflonMichel HemmerBernhard S. ZamvilScott H. LalivePatrice 2010 <p>After six months, mice were immunized with MOG p35-55 in CFA and injected i.v. with 300 ng PTx in PBS immediately following the immunization and 48 h later. 50 days after EAE induction, isolated splenocytes from 4 (control-treated group) and 6 (PTx-treated group) representative mice were cultured with anti-CD3 (0,5 µg/ml) and anti-CD28 (1 µg/ml) (<b>a–d</b>), MOG p35-55 (<b>e–h</b>) or PTx (<b>i–l</b>) and evaluated for proliferation (<b>a</b>, <b>e</b>, <b>i</b>), secretion of IFN-γ (<b>b</b>, <b>f</b>, <b>j</b>), TNF (<b>c</b>, <b>g</b>, <b>k</b>) and IL-10 (<b>d</b>, <b>h</b>, <b>l</b>) (* indicates p<0.05, ** p<0.001, *** p<0.0001; representative for two separate experiments).</p>