10.1371/journal.pgen.1001279.g004
Elin Grundberg
Elin
Grundberg
Veronique Adoue
Veronique
Adoue
Tony Kwan
Tony
Kwan
Bing Ge
Bing
Ge
Qing Ling Duan
Qing
Ling Duan
Kevin C. L. Lam
Kevin
C. L. Lam
Vonda Koka
Vonda
Koka
Andreas Kindmark
Andreas
Kindmark
Scott T. Weiss
Scott
T. Weiss
Kelan Tantisira
Kelan
Tantisira
Hans Mallmin
Hans
Mallmin
Benjamin A. Raby
Benjamin
A. Raby
Olle Nilsson
Olle
Nilsson
Tomi Pastinen
Tomi
Pastinen
DEX–specific cis-regulation of MYO6 expression.
Public Library of Science
2011
cis-regulation
myo6
2011-01-20 01:32:24
Figure
https://plos.figshare.com/articles/figure/_DEX_8211_specific_cis_regulation_of_MYO6_expression_/475544
<p>(A) The rs646967 SNP located upstream of the <i>MYO6</i> gene was shown to be strongly associated with <i>MYO6</i> expression in DEX-treated cells (top left) with no effect in BMP-2 (top middle), PGE-2 (top right) or untreated samples (data not shown). Expression scores from Illumina Ref8 BeadArrays for each individual are averaged from two biological replicates and shown as red circles and the regression line from linear regression model is indicated with blue dashes. (B) The treatment-specific effect of <i>cis</i>-regulation of <i>MYO6</i> expression was validated by sequencing-based allelic expression test in four samples heterozygous for both the rs646967 <i>cis</i>-eSNP and for the intragenic rs12606 marker. Normalized rs12606 heterozygote allele ratios of RNA samples from DEX (24h), BMP-2 (2h) and PGE<sub>2</sub> (24h) treated cells were compared within and between samples and data are presented as mean ± SD of three technical replicates. Significant P-value is obtained from t-test comparing |Δ het ratio| in DEX versus BMP-2 and PGE<sub>2</sub> samples. (C) The <i>cis</i>-eQTL analysis of the <i>MYO6</i> region was expanded by the inclusion of imputed untyped HapMapII SNPs. The P-values from the linear regression analysis represented as −log<sub>10</sub>(P-value) are shown as vertical bars with a horizontal line indicating a cutoff of P = 10<sup>−4</sup>. The rs584677 SNP, marked with an arrow, located ∼160kb upstream of the transcription start site showed the most significant association with <i>MYO6</i> expression.</p>