Galdeano, Diogo Manzano Breton, Michèle Claire Lopes, João Roberto Spotti W. Falk, Bryce Machado, Marcos Antonio Knockdown of endogenous psyllid mRNAs by dsRNA feeding on artificial diets after 120 h. <p>The dsRNAs were added to the artificial diets at concentrations of 200, 500 and 1000 ng.μL<sup>-1</sup>, and total RNAs were isolated from live psyllids after 120 h of feeding. GFP dsRNA-fed <i>D</i>. <i>citri</i> served as controls for each experiment. (A) Accumulation of <i>cathepsin D</i> mRNAs in whole psyllids fed <i>cathepsin D</i> dsRNAs at 200, 500 and 1000 ng.μL<sup>-1</sup>. (B) Accumulation of <i>chitin synthase</i> mRNAs in whole psyllids fed <i>chitin synthase</i> dsRNAs at 200, 500 and 1000 ng.μL<sup>-1</sup>. (A) Accumulation of <i>inhibitor of apoptosis</i> mRNAs in whole psyllids fed <i>inhibitor of apoptosis</i> dsRNAs at 200, 500 and 1000 ng.μL<sup>-1</sup>. Significant differences in relative expression were evaluated between test dsRNAs and GFP dsRNA at the same concentrations. A single asterisk indicates P<0.05, and double asterisks indicate P<0.01.</p> RNA interference;Murraya paniculata;dsRNA-mediated RNAi effects;Oral delivery;Adult ACP mortality;gene function studies;Candidatus Liberibacter asiaticus;HLB control;chitin synthase;phloem-limited bacteria Candidatus Liberibacter americanus;adult ACP;chemical control;cathepsin D;qRT-PCR analysis;control pests;target mRNA knockdown;Diaphorina citri;double-stranded RNA;nymph ACP;dsRNAs solutions;gene functions;disease huanglongbing;apoptosis genes;target mRNAs;double-stranded RNAs 2017-03-10
    https://plos.figshare.com/articles/figure/Knockdown_of_endogenous_psyllid_mRNAs_by_dsRNA_feeding_on_artificial_diets_after_120_h_/4744108
10.1371/journal.pone.0171847.g004