10.1371/journal.pone.0172571.g008
Li-Fu Li
Li-Fu
Li
Chung-Shu Lee
Chung-Shu
Lee
Chang-Wei Lin
Chang-Wei
Lin
Ning-Hung Chen
Ning-Hung
Chen
Li-Pang Chuang
Li-Pang
Chuang
Chen-Yiu Hung
Chen-Yiu
Hung
Yung-Yang Liu
Yung-Yang
Liu
Reduction of lung stretch-induced collagen gene expression and epithelial-mesenchymal transition in Akt-deficient mice.
Public Library of Science
2017
HDAC 4
staining
lung injury
ventilator-induced lung injury
plasminogen activator inhibitor
high-V T MV
HDAC 4 expression
MV-augmented lung inflammation
Akt-deficient C 57BL mice
TSA treatment attenuates high-V T MV-augmented EMT
ALI
MMP
Akt activation
MV-augmented bleomycin-induced EMT
attenuates ventilation-augmented epithelial-mesenchymal transition
Akt pathway Background Mechanical ventilation
1 gene expression
ARDS
matrix metalloproteinase -9
Akt pathways
2017-02-24 19:17:43
Figure
https://plos.figshare.com/articles/figure/Reduction_of_lung_stretch-induced_collagen_gene_expression_and_epithelial-mesenchymal_transition_in_Akt-deficient_mice_/4693486
<p>After five days of bleomycin treatment, real-time polymerase chain reaction of fibronectin (A), MMP-9 (B), and 1a1 (C) mRNA expression were from the lungs of nonventilated control mice and mice ventilated at a tidal volume of 30 mL/kg for 5 h with room air (n = 5 per group). Arbitrary units were expressed as the ratio of fibronectin, MMP-9, and 1a1 mRNA to GAPDH (n = 5 per group). Representative photomicrographs (x400) with E-cad (red), α-SMA (red), and Hoechst (blue) immunofluorescent staining of paraffin lung sections (D) (n = 5 per group), and micro-computed tomography imaging (E) (n = 3 per group) five days after administering bleomycin were from nonventilated control mice and mice ventilated at a tidal volume of 30 mL/kg for 5 h with room air. Positive red staining in the fibroblasts is identified by arrows (n = 5 per group). The fibrotic scoring (F) was quantified as the average number of 10 nonoverlapping fields in Masson’s trichrome staining of paraffin lung sections (n = 5 per group). Scale bars represent 20 μm. ANOVA was used to assess the statistical significance of the differences, followed by multiple comparisons with a Scheffeˊs test, and a P value < 0.05 was considered statistically significant. * P< 0.05 versus the nonventilated control mice with bleomycin pretreatment; <b>†</b>P< 0.05 versus Akt<sup>+/-</sup> group.</p>