ATG10 is involved in p62-regulated autophagy. Chunjuan Song Sayak K. Mitter Xiaoping Qi Eleni Beli Haripriya V. Rao Jindong Ding Colin S. Ip Hongmei Gu Debra Akin William A. Dunn Jr. Catherine Bowes Rickman Alfred S. Lewin Maria B. Grant Michael E. Boulton 10.1371/journal.pone.0171940.g008 https://plos.figshare.com/articles/figure/ATG10_is_involved_in_p62-regulated_autophagy_/4678498 <p><b>(A)</b> Scatter plots comparing the normalized, relative expression of autophagy-related proteins in ARPE-19 cells transfected with p62 specific or scrambled siRNA (10nM) for 24 h. mRNA expression was detected using autophagy qRT-PCR array from SA Biosciences each. The 'fold-change boundary' denoted by the parallel lines flanking the median line was set at 2 and segregates the genes up or down regulated. Dots represent genes plotted on the basis of Log <sub>10</sub>(2<sup>-ΔΔCt</sup>). Blue circles denote the genes ATG10 and p62 with -2.44 and -6.77 fold changes respectively. <b>(B)</b> p62 mRNA level was examined by qRT-PCR to confirm the knockdown of p62 transcription and mRNA level of autophagic protein ATG10 was assessed in p62-knockdown cells. <b>(C)</b> RPE cells were treated by 400μM H<sub>2</sub>O<sub>2</sub> and ATG10 mRNA level was measured by qRT-PCR. Mean+ S.E.M. from three independent experiments were plotted. <b>(D)</b> RPE cells were transfected with p62 or scrambled siRNA for 24h and then exposed with 400 H<sub>2</sub>O<sub>2</sub> for 1, 3, 6, 12h respectively. Whole-cells lysates were analyzed by Western blot with anti-p62, ATG10, or β–actin antibodies. Differences between means were considered statistically significant when p<0.05 by Mann-Whitney U-test.</p> 2017-02-21 19:15:00 modulating ATG 10 expression autophagy p 62 ATG 10 expression Reactive oxygen species NF κB p 65 p 62 NF κB gene expression p 62 upregulation APOE CM-H ROS H 2O causes p 62-NF RPE cell line ARPE -19 p 62 expression p 62. Nuclear NF κB p 65 activity RNA p 65 phosphorylation epithelial cell survival oxidative stress H 2 O 2 oxidative damage 2DCFDA apolipoprotein E 4 targeted-replacement p 62 protein HFC AMD mouse model MTT RPE cells