Assessment of Akr1c3 and Akr1b10 and γ-tubulin expression in various stable cell lines using immunblotting approaches. Simon Chewchuk Baoqing Guo Amadeo Mark Parissenti 10.1371/journal.pone.0172244.g001 https://plos.figshare.com/articles/figure/Assessment_of_Akr1c3_and_Akr1b10_and_-tubulin_expression_in_various_stable_cell_lines_using_immunblotting_approaches_/4652035 <p><b>(A)</b> Representative immunoblots for assessing Akr1c3, Akr1b10 and γ-tubulin protein expression in extracts of unselected MCF-7<sub>CC</sub> cells or doxorubicin-selected MCF-7<sub>DOX2</sub> cells (selection doses 7 through 12). Blots represent one 4 independent experiments. <b>(B)</b> Fold change in Akr1C3 levels relative to corresponding co-cultured control cell lines for doxorubicin-selected cell lines across selection doses 7 through 12 (based by densitometry). Fold changes are expressed as the average ± S.E.M. for 4 independent experiments. The significance of differences in Akr1c3 expression between the designated doxorubicin-elected cell line and its corresponding co-cultured control cell line was assessed using an ANOVA test, followed by a Bonferoni correction. * p< 0.05, ** p< 0.01, *** p< 0.001, **** p< 0.0001.</p> 2017-02-14 19:22:14 doxorubicin resistance breast tumor cells Intrinsic control MCF -7 CC cells AKR 1C gene transcript level cyclin D 1 expression E 2 synthesis AKR 1B overexpression cell proliferation experiments E 2-dependent control AKR overexpression acts MCF -7 breast tumor cells E 2 production E 2-dependent expression cell cycle-dependent kinases MCF -7 CC cells E 2-dependent pathways ER anthracycline chemotherapy drugs E 2-related genes E 2 pathways Bcl -2 anthracycline resistance MCF -7 DOX 2 protein MCF -7 EPI cells breast tumor cells AKR expression