Observed bias in amplification-based sequencing.
Lee T. Sam
Doron Lipson
Tal Raz
Xuhong Cao
John Thompson
Patrice M. Milos
Dan Robinson
Arul M. Chinnaiyan
Chandan Kumar-Sinha
Christopher A. Maher
10.1371/journal.pone.0017305.g001
https://plos.figshare.com/articles/figure/_Observed_bias_in_amplification_based_sequencing_/465128
<p>(A) Single-best mapping method-based quantile-quantile plot demonstrates evidence of over-representation of highly expressed transcripts in amplification-based sequencing compared to single-molecule methods. (B) Distribution of reads across genes by transcript concentration shows decreased SMS coverage of the most highly expressed genes, with those reads going to mid- and low-level expressors. (C) Differences in the distribution of reads lead to increased sensitivity of low-expressing transcripts. (D) Nine of the candidate genes seen above the 0.3 RPKM noise level demonstrated any amplification by RT-PCR, although only <i>HIST1H4C</i> showed high abundance.</p>
2011-03-01 01:25:28
amplification-based