Validation of the expression levels of 3 genes. Hailing Zhao Xin Li Tingting Zhao Haojun Zhang Meihua Yan Xi Dong Pengmin Chen Liang Ma Ping Li 10.1371/journal.pone.0171475.g003 https://plos.figshare.com/articles/figure/Validation_of_the_expression_levels_of_3_genes_/4636897 <p>(A) The relative expression levels of 3 genes were normalized to that of β-actin. (B) Western blot analysis was used to validate the expression level of PLZF in the 3 groups. (C) Immunohistochemistry and semi-quantitative analysis for PLZF. The data were expressed as the mean ± SEM. #P<0.05, ###P<0.001 vs. db/m group; *P<0.05, **P<0.01 vs. db/db group.</p> 2017-02-09 18:34:38 PLZF expression DKD db granule Tangshen Formula autophagy HG NRK 52E cells microarray-based gene expression collagen III TSF treatment collagen III protein injury DN Tangshen formula attenuates downregulated LC 3 II