10.1371/journal.pone.0171475.g003 Hailing Zhao Hailing Zhao Xin Li Xin Li Tingting Zhao Tingting Zhao Haojun Zhang Haojun Zhang Meihua Yan Meihua Yan Xi Dong Xi Dong Pengmin Chen Pengmin Chen Liang Ma Liang Ma Ping Li Ping Li Validation of the expression levels of 3 genes. Public Library of Science 2017 PLZF expression DKD db granule Tangshen Formula autophagy HG NRK 52E cells microarray-based gene expression collagen III TSF treatment collagen III protein injury DN Tangshen formula attenuates downregulated LC 3 II 2017-02-09 18:34:38 Figure https://plos.figshare.com/articles/figure/Validation_of_the_expression_levels_of_3_genes_/4636897 <p>(A) The relative expression levels of 3 genes were normalized to that of β-actin. (B) Western blot analysis was used to validate the expression level of PLZF in the 3 groups. (C) Immunohistochemistry and semi-quantitative analysis for PLZF. The data were expressed as the mean ± SEM. #P<0.05, ###P<0.001 vs. db/m group; *P<0.05, **P<0.01 vs. db/db group.</p>