10.1371/journal.pone.0170812.g007 Benjamin J. Dickson Benjamin J. Dickson Mohamed I. Gatie Mohamed I. Gatie Danielle M. Spice Danielle M. Spice Gregory M. Kelly Gregory M. Kelly <i>Nox</i> overexpression activates canonical Wnt signaling, but does not promote differentiation. Public Library of Science 2017 knockdown Nox 1 NOX 4 levels PrE mouse F 9 cells H 2 O 2 treatment ROS GATA F 9 cells reactive oxygen species Nox 4 show NADPH antioxidants attenuates differentiation RA NOX 1-specific inhibitors F 9 cells overexpressing Gata 6 extraembryonic endoderm Mouse F 9 cells NOX 1 NOX 4 protein levels increase 2017-02-02 18:39:20 Figure https://plos.figshare.com/articles/figure/_i_Nox_i_overexpression_activates_canonical_Wnt_signaling_but_does_not_promote_differentiation_/4612027 <p><b>(A)</b> Dual-luciferase assay of TCF activity in F9 cells transfected with <i>EV</i> and treated with DMSO or RA, or transfected with pcDNA3.1-<i>Nox1</i> or pcDNA3.1-<i>Nox4</i>. <b>(B)</b> <i>Dab2</i> expression in F9 cells transfected with <i>EV</i> and treated with DMSO or RA, or transfected with pcDNA3.1-<i>Nox1</i>. <b>(C)</b> <i>Dab2</i> expression in F9 cells transfected with <i>EV</i> and treated with DMSO or RA, or transfected with pcDNA3.1-<i>Nox4</i>. <b>(D)</b> Immunoblot analysis for DAB2, TROMA1, and OCT4 in F9 cells transfected with <i>EV</i> and treated with DMSO or RA, or transfected with pcDNA3.1-<i>Nox1</i>, pcDNA3.1-<i>Nox4</i> or both vectors. β-actin was used as a loading control. A total of 3 independent experiments were analyzed and results are presented as mean ± SEM. Letters denote groups of significance (<i>P</i> < 0.05) tested by a One-Way ANOVA followed by a Tukey’s test.</p>