10.1371/journal.pone.0170812.g007
Benjamin J. Dickson
Benjamin J.
Dickson
Mohamed I. Gatie
Mohamed I.
Gatie
Danielle M. Spice
Danielle M.
Spice
Gregory M. Kelly
Gregory
M. Kelly
<i>Nox</i> overexpression activates canonical Wnt signaling, but does not promote differentiation.
Public Library of Science
2017
knockdown Nox 1
NOX 4 levels
PrE
mouse F 9 cells
H 2 O 2 treatment
ROS
GATA
F 9 cells
reactive oxygen species
Nox 4 show
NADPH
antioxidants attenuates differentiation
RA
NOX 1-specific inhibitors
F 9 cells overexpressing Gata 6
extraembryonic endoderm Mouse F 9 cells
NOX 1
NOX 4 protein levels increase
2017-02-02 18:39:20
Figure
https://plos.figshare.com/articles/figure/_i_Nox_i_overexpression_activates_canonical_Wnt_signaling_but_does_not_promote_differentiation_/4612027
<p><b>(A)</b> Dual-luciferase assay of TCF activity in F9 cells transfected with <i>EV</i> and treated with DMSO or RA, or transfected with pcDNA3.1-<i>Nox1</i> or pcDNA3.1-<i>Nox4</i>. <b>(B)</b> <i>Dab2</i> expression in F9 cells transfected with <i>EV</i> and treated with DMSO or RA, or transfected with pcDNA3.1-<i>Nox1</i>. <b>(C)</b> <i>Dab2</i> expression in F9 cells transfected with <i>EV</i> and treated with DMSO or RA, or transfected with pcDNA3.1-<i>Nox4</i>. <b>(D)</b> Immunoblot analysis for DAB2, TROMA1, and OCT4 in F9 cells transfected with <i>EV</i> and treated with DMSO or RA, or transfected with pcDNA3.1-<i>Nox1</i>, pcDNA3.1-<i>Nox4</i> or both vectors. β-actin was used as a loading control. A total of 3 independent experiments were analyzed and results are presented as mean ± SEM. Letters denote groups of significance (<i>P</i> < 0.05) tested by a One-Way ANOVA followed by a Tukey’s test.</p>