10.1371/journal.pone.0170812.g005
Benjamin J. Dickson
Benjamin J.
Dickson
Mohamed I. Gatie
Mohamed I.
Gatie
Danielle M. Spice
Danielle M.
Spice
Gregory M. Kelly
Gregory
M. Kelly
<i>Nox1</i> and <i>Nox4</i> knockdown attenuates RA-induced differentiation.
Public Library of Science
2017
knockdown Nox 1
NOX 4 levels
PrE
mouse F 9 cells
H 2 O 2 treatment
ROS
GATA
F 9 cells
reactive oxygen species
Nox 4 show
NADPH
antioxidants attenuates differentiation
RA
NOX 1-specific inhibitors
F 9 cells overexpressing Gata 6
extraembryonic endoderm Mouse F 9 cells
NOX 1
NOX 4 protein levels increase
2017-02-02 18:39:20
Figure
https://plos.figshare.com/articles/figure/_i_Nox1_i_and_i_Nox4_i_knockdown_attenuates_RA-induced_differentiation_/4612003
<p>RNA was collected from F9 cells transfected with scrambled (<i>scr</i>) si-<i>RNA</i>, si-<i>Nox1</i> and/or si-<i>Nox4</i> siRNA and cultured 4 days with RA treatment. <b>(A)</b> <i>Dab2</i> expression of F9 cells transfected with <i>scr</i>, si<i>-Nox1</i>, or si<i>-Nox4</i> and treated with RA. <b>(B)</b> Immunoblot analysis for DAB2, TROMA1, and OCT4 in F9 cells transfected with <i>scr</i>, si<i>-Nox1</i>, or si<i>-Nox4</i> and treated with RA. <b>(C)</b> Densitometric analysis for DAB2, TROMA1, and <b>(D)</b> OCT4 in F9 cells transfected with <i>scr</i>, si<i>-Nox1</i>, or si<i>-Nox4</i> and treated with RA. β-actin was used as a loading control. A total of 3 independent experiments were analyzed and results are presented as mean ± SEM. Letters and symbols denote groups of significance (<i>P</i> < 0.05) tested by a One-Way ANOVA followed by a Tukey’s test.</p>