10.1371/journal.pone.0170812.g005 Benjamin J. Dickson Benjamin J. Dickson Mohamed I. Gatie Mohamed I. Gatie Danielle M. Spice Danielle M. Spice Gregory M. Kelly Gregory M. Kelly <i>Nox1</i> and <i>Nox4</i> knockdown attenuates RA-induced differentiation. Public Library of Science 2017 knockdown Nox 1 NOX 4 levels PrE mouse F 9 cells H 2 O 2 treatment ROS GATA F 9 cells reactive oxygen species Nox 4 show NADPH antioxidants attenuates differentiation RA NOX 1-specific inhibitors F 9 cells overexpressing Gata 6 extraembryonic endoderm Mouse F 9 cells NOX 1 NOX 4 protein levels increase 2017-02-02 18:39:20 Figure https://plos.figshare.com/articles/figure/_i_Nox1_i_and_i_Nox4_i_knockdown_attenuates_RA-induced_differentiation_/4612003 <p>RNA was collected from F9 cells transfected with scrambled (<i>scr</i>) si-<i>RNA</i>, si-<i>Nox1</i> and/or si-<i>Nox4</i> siRNA and cultured 4 days with RA treatment. <b>(A)</b> <i>Dab2</i> expression of F9 cells transfected with <i>scr</i>, si<i>-Nox1</i>, or si<i>-Nox4</i> and treated with RA. <b>(B)</b> Immunoblot analysis for DAB2, TROMA1, and OCT4 in F9 cells transfected with <i>scr</i>, si<i>-Nox1</i>, or si<i>-Nox4</i> and treated with RA. <b>(C)</b> Densitometric analysis for DAB2, TROMA1, and <b>(D)</b> OCT4 in F9 cells transfected with <i>scr</i>, si<i>-Nox1</i>, or si<i>-Nox4</i> and treated with RA. β-actin was used as a loading control. A total of 3 independent experiments were analyzed and results are presented as mean ± SEM. Letters and symbols denote groups of significance (<i>P</i> < 0.05) tested by a One-Way ANOVA followed by a Tukey’s test.</p>