Ketamine-induced cell death is counteracted by PACAP <i>via</i> PAC-1 receptor activation. Shiva Mansouri Ingrid Agartz Sven-Ove Ögren Cesare Patrone Mathias Lundberg 10.1371/journal.pone.0170496.g003 https://plos.figshare.com/articles/figure/Ketamine-induced_cell_death_is_counteracted_by_PACAP_i_via_i_PAC-1_receptor_activation_/4605382 <p>NSCs were plated as single cells. Cells were incubated with 400 μM ketamine and PACAP (100 nM) or agonist for PAC-1; Max-4 (30 nM). After 24 hour incubation, intracellular ATP levels were measured for cell viability. Data are shown as mean ±SEM (n = 13–39). Kruskal-Wallis followed by Dunn’s test was used. Differences were considered significant at P<0.05. * denotes P<0.05 compared with control, # denotes P<0.05 compared to 400 μM ketamine.</p> 2017-01-26 00:38:59 RT-PCR AMPA pathway activation mTOR Pathway Activation Ketamine administration ketamine impairs NSC viability schizophrenia Western blot analysis NSC viability ER AMPA receptor activation Adult neurogenesis dysregulation PACAP Protects Adult Neural Stem Cells adenylate cyclase-activating polypeptide