10.1371/journal.ppat.1006181.g003
Jason E. Hammonds
Jason E.
Hammonds
Neal Beeman
Neal
Beeman
Lingmei Ding
Lingmei
Ding
Sarah Takushi
Sarah
Takushi
Ashwanth C. Francis
Ashwanth C.
Francis
Jaang-Jiun Wang
Jaang-Jiun
Wang
Gregory B. Melikyan
Gregory
B. Melikyan
Paul Spearman
Paul
Spearman
Siglec-1 RNAi interference and competitive inhibition by a GM3 glycan mimetic inhibits HIV-1 VLP internalization by MDMs.
Public Library of Science
2017
Siglec -1-mediated virion
particle uptake
macrophage-to-T cell transmission
virus-containing compartment
VCC formation
HIV
cell surface lectin
non-infectious virus-like particles
intracellular compartment
target T lymphocytes
autologous T cells
ganglioside-containing virus-like particles
2017-01-27 04:30:02
Figure
https://plos.figshare.com/articles/figure/Siglec-1_RNAi_interference_and_competitive_inhibition_by_a_GM3_glycan_mimetic_inhibits_HIV-1_VLP_internalization_by_MDMs_/4592767
<p><b>(A)</b> MDMs were transfected with 60 nM control or Siglec-1 siRNA on day 8 after plating. Cell lysates were harvested and analyzed by Western blotting for Siglec-1 and actin expression. <b>(B)</b> MDMs were transfected with either control or Siglec-1 siRNA and 5 days later incubated with 400 ng of HIV-1 Gag-EGFP VLPs for 2 hr. Cells were washed and cell lysate p24 concentration determined by ELISA. <b>(C)</b> MDMs were pre-treated at RT with lactose or 3’-sialyllactose for 1 hour. Subsequently, MDMs were incubated with 400 ng of HIV-1 Gag-EGFP VLPs in the presence of compound for an additional hour. MDMs were then washed, cell lysates harvested and cell-associated p24 measured by ELISA. Error bars represent standard deviation; asterisks depict significant differences as measured by unpaired <i>t</i>-test.</p>