10.1371/journal.ppat.1006181.g003 Jason E. Hammonds Jason E. Hammonds Neal Beeman Neal Beeman Lingmei Ding Lingmei Ding Sarah Takushi Sarah Takushi Ashwanth C. Francis Ashwanth C. Francis Jaang-Jiun Wang Jaang-Jiun Wang Gregory B. Melikyan Gregory B. Melikyan Paul Spearman Paul Spearman Siglec-1 RNAi interference and competitive inhibition by a GM3 glycan mimetic inhibits HIV-1 VLP internalization by MDMs. Public Library of Science 2017 Siglec -1-mediated virion particle uptake macrophage-to-T cell transmission virus-containing compartment VCC formation HIV cell surface lectin non-infectious virus-like particles intracellular compartment target T lymphocytes autologous T cells ganglioside-containing virus-like particles 2017-01-27 04:30:02 Figure https://plos.figshare.com/articles/figure/Siglec-1_RNAi_interference_and_competitive_inhibition_by_a_GM3_glycan_mimetic_inhibits_HIV-1_VLP_internalization_by_MDMs_/4592767 <p><b>(A)</b> MDMs were transfected with 60 nM control or Siglec-1 siRNA on day 8 after plating. Cell lysates were harvested and analyzed by Western blotting for Siglec-1 and actin expression. <b>(B)</b> MDMs were transfected with either control or Siglec-1 siRNA and 5 days later incubated with 400 ng of HIV-1 Gag-EGFP VLPs for 2 hr. Cells were washed and cell lysate p24 concentration determined by ELISA. <b>(C)</b> MDMs were pre-treated at RT with lactose or 3’-sialyllactose for 1 hour. Subsequently, MDMs were incubated with 400 ng of HIV-1 Gag-EGFP VLPs in the presence of compound for an additional hour. MDMs were then washed, cell lysates harvested and cell-associated p24 measured by ELISA. Error bars represent standard deviation; asterisks depict significant differences as measured by unpaired <i>t</i>-test.</p>