Metformin inhibits grade 1 endometrial cancer cell proliferation, survival, migration, and induces apoptosis.
John J. Wallbillich
Srirama Josyula
Uksha Saini
Roman A. Zingarelli
Kalpana Deepa Priya Dorayappan
Maria K. Riley
Ross A. Wanner
David E. Cohn
Karuppaiyah Selvendiran
10.1371/journal.pone.0170318.g004
https://plos.figshare.com/articles/figure/Metformin_inhibits_grade_1_endometrial_cancer_cell_proliferation_survival_migration_and_induces_apoptosis_/4583380
<p><b>A</b>. Sulforhodamine B (SRB) assay measured Ishikawa cell proliferation with increasing concentrations of metformin after 24h or 48h, in high-glucose media. <b>B</b>. Proportion of Ishikawa cells surviving (compared to control) after treatment with 10 mM or 20 mM of metformin. <b>C</b>. Cell migration assay, with Ishikawa cells subjected to control, 10 mM, or 20 mM metformin for 24h. Control (0h) is also shown. <b>D</b>. Quantification of % wound closure; the 10 mM and 20 mM treatment groups were each significantly different than control (p<0.05; noted with asterisk [*]). <b>E</b>. Western blot of apoptosis-related protein expression in Ishikawa cells after treatment with control, 10 mM, or 20 mM for 48h.</p>
2017-01-23 17:39:28
STAT 3 overexpression plasmid
target proteins
High Glucose-Mediated STAT 3 Activation
Ishikawa cells transfected
Endometrial Cancer Objectives STAT 3
xenograft tumor model
Western blot
type 1 endometrial cancer
apoptotic protein expression
PCR
glucose concentrations influence STAT 3 expression
STAT 3 overexpression
STAT 3
pSTAT 3 ser 727.
cell proliferation
metformin treatment
Ishikawa cells
STAT 3 expression