DOX induces HNE adduction with pJNK and Bcl-2 phosphorylation, and triggers autophagic response.
Joyce M. Velez
Sumitra Miriyala
Ramaneeya Nithipongvanitch
Teresa Noel
Chotiros D. Plabplueng
Terry Oberley
Paiboon Jungsuwadee
Holly Van Remmen
Mary Vore
Daret K. St. Clair
10.1371/journal.pone.0018005.g002
https://plos.figshare.com/articles/figure/_DOX_induces_HNE_adduction_with_pJNK_and_Bcl_2_phosphorylation_and_triggers_autophagic_response_/457780
<p>A. PhosphoJNK was detected by immunoprecipitation using a polyclonal goat anti-HNE antibody followed by Western blot analysis with a rabbit polyclonal Thr183/Tyr185 phosphorylation specific JNK antibody. Quantitative analysis represents the Mean ± SEM (n = 5) in each group; *p<0.001 as compared to other groups. B. Western blot analysis of p-Bcl2 in cardiac mitochondria from WT and p53<sup>(−/−)</sup> mice treated with saline or DOX and quantitative analysis represents the Mean ± SEM n = 5 in each group; *p<0.001 as compared to other groups. C. Western blot analysis of Beclin1 in cardiac mitochondria from WT and p53<sup>(−/−)</sup> mice treated with saline or DOX and quantitative analysis represents the Mean ± SEM n = 5 in each group; *p<0.001 as compared to other groups. D. Co-immunoprecipitation of Beclin1 with Bcl-2 in cardiac mitochondria from WT and p53<sup>(−/−)</sup> mice treated with saline or DOX and quantitative analysis represents the Mean ± SEM n = 5 in each group; *p<0.01 as compared to other groups.</p>
2013-02-20 19:32:52
induces
hne
adduction
pjnk
bcl-2
triggers
autophagic