DOX induces HNE adduction with pJNK and Bcl-2 phosphorylation, and triggers autophagic response. Joyce M. Velez Sumitra Miriyala Ramaneeya Nithipongvanitch Teresa Noel Chotiros D. Plabplueng Terry Oberley Paiboon Jungsuwadee Holly Van Remmen Mary Vore Daret K. St. Clair 10.1371/journal.pone.0018005.g002 https://plos.figshare.com/articles/figure/_DOX_induces_HNE_adduction_with_pJNK_and_Bcl_2_phosphorylation_and_triggers_autophagic_response_/457780 <p>A. PhosphoJNK was detected by immunoprecipitation using a polyclonal goat anti-HNE antibody followed by Western blot analysis with a rabbit polyclonal Thr183/Tyr185 phosphorylation specific JNK antibody. Quantitative analysis represents the Mean ± SEM (n = 5) in each group; *p<0.001 as compared to other groups. B. Western blot analysis of p-Bcl2 in cardiac mitochondria from WT and p53<sup>(−/−)</sup> mice treated with saline or DOX and quantitative analysis represents the Mean ± SEM n = 5 in each group; *p<0.001 as compared to other groups. C. Western blot analysis of Beclin1 in cardiac mitochondria from WT and p53<sup>(−/−)</sup> mice treated with saline or DOX and quantitative analysis represents the Mean ± SEM n = 5 in each group; *p<0.001 as compared to other groups. D. Co-immunoprecipitation of Beclin1 with Bcl-2 in cardiac mitochondria from WT and p53<sup>(−/−)</sup> mice treated with saline or DOX and quantitative analysis represents the Mean ± SEM n = 5 in each group; *p<0.01 as compared to other groups.</p> 2013-02-20 19:32:52 induces hne adduction pjnk bcl-2 triggers autophagic