Correlation of RNA-seq and qRT-PCR. CummingsJason E. SlaydenRichard A. 2017 <p>Fragments per kilobase of transcript per million mapped reads (FPKM) for differentially expressed genes and 16s reference gene were converted to Log2 values and plotted against respective qRT-PCR crossing point (Cp) values. Linear regression analysis (dotted line) was run to determine if the correlation coefficient was >0.7. An inverse relationship between Cp and FPKM is an indication of correlation and validation of the RNA-seq data set.</p>