%0 Figure %A C. Jham, Bruno %A Ma, Tao %A Hu, Jiadi %A Chaisuparat, Risa %A R. Friedman, Eitan %A Paolo Pandolfi, Pier %A Schneider, Abraham %A Sodhi, Akrit %A Montaner, Silvia %D 2011 %T Upregulation of HIF-1α/2α by vGPCR paracrine secretions. %U https://plos.figshare.com/articles/figure/_Upregulation_of_HIF_1_945_2_945_by_vGPCR_paracrine_secretions_/449859 %R 10.1371/journal.pone.0019103.g003 %2 https://plos.figshare.com/ndownloader/files/779493 %K vgpcr %K paracrine %X

(A) HMEC1s were exposed to media conditioned by control (Control CM) or vGPCR-expressing cells (vGPCR CM), in the presence of vehicle or Rapamycin (50 µM). mRNA levels of HIF-1α and HIF-2α and protein levels of HIF-1α, HIF-2α and VEGF are shown. (B) HMEC1s were transfected with increasing doses (20, 40 and 80 nM) of HIF-1β siRNA or Scrambled siRNA. Cells were then exposed to media conditioned by vGPCR-expressing cells. VEGF mRNA levels are shown. (C) Immunohistochemical analysis of HIF-1α and HIF-2α, and staining using an isotype-matched control antibody, in vGPCR tumor and human KS.

%I PLOS ONE