Ouabain down-regulates TGFβR2 mRNA and protein levels in human lung fibroblasts (HLF). Jennifer La Eleanor Reed Lan Chan Larisa V. Smolyaninova Olga A. Akomova Gökhan M. Mutlu Sergei N. Orlov Nickolai O. Dulin 10.1371/journal.pone.0168363.g001 https://plos.figshare.com/articles/figure/Ouabain_down-regulates_TGF_R2_mRNA_and_protein_levels_in_human_lung_fibroblasts_HLF_/4493507 <p>Serum-starved HLF were treated with 100 nM ouabain for 3, 6, and 24 hours. Cells were analyzed by real-time qPCR for TGFβR2 mRNA levels (<b>A</b>) and by Western blotting for TGFβR2 protein levels (<b>B</b>). Shown are the representative images and the quantitative analysis of ECL (mean±SD) from at least three independent experiments (RLU, relative light units). **<i>p</i> < 0.005, ***<i>p</i> < 0.0005. <b>C.</b> HLF isolated and cultured from IPF and non-IPF (NL) lungs were treated with or without 100 nM ouabain. Cells lysates were analyzed for TGFβR2 or tubulin protein expression by Western blotting.</p> 2016-12-22 18:55:25 TGF βR expression TGF -β target genes TGF -β signals integral plasma membrane protein HLF Na TGF -β receptors 1 TGF βR TGF -β-induced Smad phosphorylation lung alveolar epithelial cells