10.1371/journal.pone.0020467.g004
Satoshi Sugita
Satoshi
Sugita
Yasutomi Kamei
Yasutomi
Kamei
Fumiko Akaike
Fumiko
Akaike
Takayoshi Suganami
Takayoshi
Suganami
Sayaka Kanai
Sayaka
Kanai
Maki Hattori
Maki
Hattori
Yasuko Manabe
Yasuko
Manabe
Nobuharu Fujii
Nobuharu
Fujii
Takako Takai-Igarashi
Takako
Takai-Igarashi
Miki Tadaishi
Miki
Tadaishi
Jun-Ichiro Oka
Jun-Ichiro
Oka
Hiroyuki Aburatani
Hiroyuki
Aburatani
Tetsuya Yamada
Tetsuya
Yamada
Hideki Katagiri
Hideki
Katagiri
Saori Kakehi
Saori
Kakehi
Yoshifumi Tamura
Yoshifumi
Tamura
Hideo Kubo
Hideo
Kubo
Kenichi Nishida
Kenichi
Nishida
Shinji Miura
Shinji
Miura
Osamu Ezaki
Osamu
Ezaki
Yoshihiro Ogawa
Yoshihiro
Ogawa
Transient transfection-reporter assay of the effect of RXRγ on
<i>Glut1</i> promoter.
Public Library of Science
2011
transfection-reporter
assay
on
2011-05-31 00:09:36
Figure
https://plos.figshare.com/articles/figure/_Transient_transfection_reporter_assay_of_the_effect_of_RXR_on_____Glut1_promoter_/440576
<p>(<i>A</i>) <i>Glut1</i>-Luc plasmid, with or without
RXRγ and/or PPARδ expression vectors, was transfected into the
quadriceps muscle of C57BL6 mice. Activation of the luciferase reporter
gene was measured in relative light units and normalized to dual
luciferase activity. Mean values from experiments
(n = 5) are shown as fold induction, where the
activity in the absence of RXRγ is the reference value (set at 100).
(<i>B</i>) Schematic representations of serial deletion of
<i>Glut1</i> promoter constructs are shown in the figure.
Squares denote the putative PPAR/RXR binding sites. Open bars;
<i>Glut1</i>-Luc without RXRγ and PPARδ expression
vectors, and filled bars; <i>Glut1</i>-Luc with RXRγ and
PPARδ expression vectors. The activity in the absence of RXRγ
and PPARδ in each experiment for different
<i>Glut1</i>-Luc construct in the reference value (set at
100). ** <i>P</i><0.01, compared with the value of
wild-type promoter in the absence of RXRγ/PPARδ.</p>