10.1371/journal.pone.0020467.g004 Satoshi Sugita Satoshi Sugita Yasutomi Kamei Yasutomi Kamei Fumiko Akaike Fumiko Akaike Takayoshi Suganami Takayoshi Suganami Sayaka Kanai Sayaka Kanai Maki Hattori Maki Hattori Yasuko Manabe Yasuko Manabe Nobuharu Fujii Nobuharu Fujii Takako Takai-Igarashi Takako Takai-Igarashi Miki Tadaishi Miki Tadaishi Jun-Ichiro Oka Jun-Ichiro Oka Hiroyuki Aburatani Hiroyuki Aburatani Tetsuya Yamada Tetsuya Yamada Hideki Katagiri Hideki Katagiri Saori Kakehi Saori Kakehi Yoshifumi Tamura Yoshifumi Tamura Hideo Kubo Hideo Kubo Kenichi Nishida Kenichi Nishida Shinji Miura Shinji Miura Osamu Ezaki Osamu Ezaki Yoshihiro Ogawa Yoshihiro Ogawa Transient transfection-reporter assay of the effect of RXRγ on <i>Glut1</i> promoter. Public Library of Science 2011 transfection-reporter assay on 2011-05-31 00:09:36 Figure https://plos.figshare.com/articles/figure/_Transient_transfection_reporter_assay_of_the_effect_of_RXR_on_____Glut1_promoter_/440576 <p>(<i>A</i>) <i>Glut1</i>-Luc plasmid, with or without RXRγ and/or PPARδ expression vectors, was transfected into the quadriceps muscle of C57BL6 mice. Activation of the luciferase reporter gene was measured in relative light units and normalized to dual luciferase activity. Mean values from experiments (n = 5) are shown as fold induction, where the activity in the absence of RXRγ is the reference value (set at 100). (<i>B</i>) Schematic representations of serial deletion of <i>Glut1</i> promoter constructs are shown in the figure. Squares denote the putative PPAR/RXR binding sites. Open bars; <i>Glut1</i>-Luc without RXRγ and PPARδ expression vectors, and filled bars; <i>Glut1</i>-Luc with RXRγ and PPARδ expression vectors. The activity in the absence of RXRγ and PPARδ in each experiment for different <i>Glut1</i>-Luc construct in the reference value (set at 100). ** <i>P</i><0.01, compared with the value of wild-type promoter in the absence of RXRγ/PPARδ.</p>