Asymmetric expression and function of <i>hlh-14</i> in the C-lineage. J. PooleRichard BashllariEnkelejda CochellaLuisa B. FlowersEileen HobertOliver 2011 <p>A: Ventral view of the 3D-positions of certain cells of the C-lineage of a representative wild-type embryo at the AB<sup>256</sup>-cell stage (shortly after the division of the ASE grandmothers), as assessed by 4D-lineage analysis. The left-sided asymmetric neuroblast Caapa and the neuron PVR express <i>hlh-14</i> (purple asterisks). Cells of the gut and the ASE lineages are shown for positional information. Cell coloring, except C-lineage, after <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002109#pgen.1002109-Schnabel1" target="_blank">[10]</a>. B: Ventral view in both DIC and fluorescence of a representative lineaged <i>hlh-14(tm295</i>); Ex[<i>hlh-14<sup>FOS</sup>::yfp</i>]; <i>ntIs1</i> embryo at the AB<sup>256</sup>-cell stage. C: Panel shows the expression (purple lines) of <i>hlh-14</i> and the division pattern of part of the C-lineage as determined by 4D-lineage analysis of wild-type (containing the <i>ntIs1</i> transgene, <i>hlh-14(gm34); otIs114</i>, <i>hlh-14(tm295); ntIs1</i> and <i>hlh-14(tm295); otEx4507</i>, Ex<i>[hlh-14<sup>FOS</sup>::yfp]; ntIs1</i> embryos. <i>hlh-14</i> expression was assessed in both <i>gmIs20</i> and <i>hlh-14(tm295); Ex[hlh-14<sup>FOS</sup>::yfp]</i> animals. The Caapa blastomere divides early in <i>hlh-14</i> mutants, at a time when closely related cells produce hypodermal cells. This defect is rescued by the <i>hlh-14<sup>FOS</sup>::yfp</i> transgene. The extra hyp11 cell that can be observed expressing <i>dpy-7</i> (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002109#pgen.1002109.s003" target="_blank">Figure S3</a>), is indicated. The number of lineages that divided as indicated and the total number of individual left or right lineages followed to terminal division are shown at the bottom of each lineage diagram. Red cell names indicate neurons/glia and yellow cell names indicate hypodermis.</p>