Hellmann, Janina Kristin Münter, Sylvia Kudryashev, Mikhail Schulz, Simon Heiss, Kirsten Müller, Ann-Kristin Matuschewski, Kai Spatz, Joachim P. S. Schwarz, Ulrich Frischknecht, Friedrich Different patterns of <i>P. berghei</i> sporozoite migration in the skin. <p>(A) Sporozoites gliding within the skin of the ear. i: Colored tracks of 6 gliding sporozoites over 275 s (see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002080#ppat.1002080.s006" target="_blank">Video S1</a>) . Scale bar: 25 µm. ii: single frames and maximum fluorescent intensity projection of an immotile and a circular gliding sporozoite. Scale bar: 10 µm. iii: projection of a meandering sporozoite (left: max projection over 82 s; right: pseudo-colored track (RGB) of three different time periods of the same acquisition). Time is indicated in seconds. (B) Sporozoites gliding within the skin of the tail. i: Colored tracks of 8 gliding parasite over 350 s (see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002080#ppat.1002080.s006" target="_blank">Video S1</a>). Scale bar: 25 µm. ii: projection of three different time periods within one acquisition and the pseudo-colored merge (RGB) of these projections. White arrowheads point to the same parasite during the acquisition; black arrowheads point to the paths of other parasites. Time is indicated in seconds. (C) Automatically detected motility patterns of sporozoites imaged in the skin of the ear (n = 589) and tail (n = 164) showing a preference for meandering and linear movement patterns in the ear and tail, respectively, *** indicates P<0.001, motility patterns: nm = non motile; c = circling; l = linear; m = meandering. (D) Speed plot showing the median speed distribution of all tracked parasites imaged at 0.2 Hz in the dermis of ear or tail; whiskers and box indicate 97.5% and 50% of all values, respectively; horizontal bar: median (ear: 0.91 µm/s; tail: 1.10 µm/s; * indicates P<0.05).</p> patterns;sporozoite 2011-06-16
    https://plos.figshare.com/articles/figure/_Different_patterns_of_P_berghei_sporozoite_migration_in_the_skin_/435562
10.1371/journal.ppat.1002080.g001