10.1371/journal.pone.0020832.g001 Alessandro Marsili Alessandro Marsili Cristina Aguayo-Mazzucato Cristina Aguayo-Mazzucato Ting Chen Ting Chen Aditi Kumar Aditi Kumar Mirra Chung Mirra Chung Elaine P. Lunsford Elaine P. Lunsford John W. Harney John W. Harney Thuy Van-Tran Thuy Van-Tran Elena Gianetti Elena Gianetti Waile Ramadan Waile Ramadan Cyril Chou Cyril Chou Susan Bonner-Weir Susan Bonner-Weir Philip Reed Larsen Philip Reed Larsen Jorge Enrique Silva Jorge Enrique Silva Ann Marie Zavacki Ann Marie Zavacki Male D2KO mice weight gain and body fat on a HFD. Public Library of Science 2011 d2ko mice 2011-06-16 01:17:44 Figure https://plos.figshare.com/articles/figure/_Male_D2KO_mice_weight_gain_and_body_fat_on_a_HFD_/434664 <p>(A) Body weight of wild type and D2KO mice on chow or a HFD for 6-weeks, n = 22–24 mice/genotype for HFD group, n = 5 mice/genotype for chow group. A significant interaction between genotype and diet were determined by two-way ANOVA for repeated measures (p<0.001; F = 33.55; Df = 312). After Bonferroni correction, there was a significant difference in weight gain of D2KO vs. WT after 4, 5 and 6 weeks of diet (p<0.05, 0.01 and 0.001). (B) Weight gain expressed as % of initial weight after 6 weeks on either chow or a HFD of mice shown in (A). Two-way ANOVA indicated a significant interaction between genotype and diet (2WA g×d) (p<0.05; F = 7.11; Df = 52). (C) % body fat as determined by microCT, n = 4–5 mice/group. Two-way ANOVA indicated a significant interaction between genotype and diet (p<0.05; F = 4.87; Df = 15). (D) Density of voxels falling in the Hounsfield Unit range defined for adipose tissue of a representative wild type and D2KO mouse. Red is more dense while purple is less in the colorbar range. (E) Average food intake of wild type and D2KO mice on either a chow or HFD was monitored for 7 days at 3 weeks of diet and is shown expressed as kcal/day/mouse. Two-way ANOVA indicated that diet significantly effected the overall caloric consumption independent of genotype (2WA d) (p<0.01; F = 10.25; Df = 16). When a significant interaction between genotype and diet was found individual means were compared within groups by unpaired Student's t-test. Data presented are the mean ± SEM; * = p<0.05, ** = p<0.01, *** = p<0.001, ns = not significant.</p>