Wolny, Marcin Grzybek, Michał Bok, Ewa Chorzalska, Anna Lenoir, Marc Czogalla, Aleksander Adamczyk, Klaudia Kolondra, Adam Diakowski, Witold Overduin, Michael F. Sikorski, Aleksander Interaction of recombinant ankyrin-binding domain (ankBDn) and its mutants with PE/PC lipid monolayer. <p>A) Interaction of ankBDn and its mutants with PE/PC 3∶2 (w/w) monolayer. The first 38 amino acid residues of ankBDn are shown; substitutions are marked in red. The ankBDn plateau level is defined as 100% PE/PC binding activity and is equal to ∼2.5 mN/m. Plateau levels were obtained at a protein concentration of ∼25–35 nM. Higher plateau levels indicate increased penetration of the lipid monolayer by protein. Average values were calculated from at least three measurements. Significant differences from the plateau value obtained for wild-type ankBDn are marked (Student's t test): * p<0.05; ** p<0.0001. Details in “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0021538#s4" target="_blank">Materials and Methods</a>”. B) Interaction of ankBDn and its quadruple mutant SSSA with PS/PC 3∶2 (w/w) monolayer. Interaction of ankBDn with PE/PC monolayer was defined as 100%.</p> recombinant;ankyrin-binding;mutants;lipid 2011-06-28
    https://plos.figshare.com/articles/figure/_Interaction_of_recombinant_ankyrin_binding_domain_ankBDn_and_its_mutants_with_PE_PC_lipid_monolayer_/431215
10.1371/journal.pone.0021538.g003