10.1371/journal.pone.0021538.g003
Marcin Wolny
Marcin
Wolny
Michał Grzybek
Michał
Grzybek
Ewa Bok
Ewa
Bok
Anna Chorzalska
Anna
Chorzalska
Marc Lenoir
Marc
Lenoir
Aleksander Czogalla
Aleksander
Czogalla
Klaudia Adamczyk
Klaudia
Adamczyk
Adam Kolondra
Adam
Kolondra
Witold Diakowski
Witold
Diakowski
Michael Overduin
Michael
Overduin
Aleksander F. Sikorski
Aleksander
F. Sikorski
Interaction of recombinant ankyrin-binding domain (ankBDn) and its mutants with PE/PC lipid monolayer.
Public Library of Science
2011
recombinant
ankyrin-binding
mutants
lipid
2011-06-28 00:20:15
Figure
https://plos.figshare.com/articles/figure/_Interaction_of_recombinant_ankyrin_binding_domain_ankBDn_and_its_mutants_with_PE_PC_lipid_monolayer_/431215
<p>A) Interaction of ankBDn and its mutants with PE/PC 3∶2 (w/w) monolayer. The first 38 amino acid residues of ankBDn are shown; substitutions are marked in red. The ankBDn plateau level is defined as 100% PE/PC binding activity and is equal to ∼2.5 mN/m. Plateau levels were obtained at a protein concentration of ∼25–35 nM. Higher plateau levels indicate increased penetration of the lipid monolayer by protein. Average values were calculated from at least three measurements. Significant differences from the plateau value obtained for wild-type ankBDn are marked (Student's t test): * p<0.05; ** p<0.0001. Details in “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0021538#s4" target="_blank">Materials and Methods</a>”. B) Interaction of ankBDn and its quadruple mutant SSSA with PS/PC 3∶2 (w/w) monolayer. Interaction of ankBDn with PE/PC monolayer was defined as 100%.</p>