China, Arnab Mishra, Sonakshi Nagaraja, Valakunja RNAP can restart transcription elongation after transcript cleavage by <i>Mtb</i>Gre. <p>The starting materials in all the experiments were purified stalled complexes with radiolabeled RNA. (<b>A</b>) Cleavage-restart assay on T7A1-TEC. The scheme on the left depicts the reaction process. The TEC was incubated without (lane 1) or with <i>Mtb</i>Gre (lane 2 and 3), or NTPs (lane 3 and 4), as indicated. (<b>B</b>) Similar assay was carried out on <i>M. smegmatis</i> P<i><sub>rnB</sub></i> promoter template (the RNAP stalls at +39 position in the absence of UTP in the reaction mix). Transcripts were analyzed by resolving on 20% urea PAGE.</p> restart;transcription;elongation;transcript;cleavage 2011-07-08
    https://plos.figshare.com/articles/figure/_RNAP_can_restart_transcription_elongation_after_transcript_cleavage_by_Mtb_Gre_/428457
10.1371/journal.pone.0021941.g005