%0 Figure %A China, Arnab %A Mishra, Sonakshi %A Nagaraja, Valakunja %D 2011 %T RNAP can restart transcription elongation after transcript cleavage by MtbGre. %U https://plos.figshare.com/articles/figure/_RNAP_can_restart_transcription_elongation_after_transcript_cleavage_by_Mtb_Gre_/428457 %R 10.1371/journal.pone.0021941.g005 %2 https://plos.figshare.com/ndownloader/files/758086 %K restart %K transcription %K elongation %K transcript %K cleavage %X

The starting materials in all the experiments were purified stalled complexes with radiolabeled RNA. (A) Cleavage-restart assay on T7A1-TEC. The scheme on the left depicts the reaction process. The TEC was incubated without (lane 1) or with MtbGre (lane 2 and 3), or NTPs (lane 3 and 4), as indicated. (B) Similar assay was carried out on M. smegmatis PrnB promoter template (the RNAP stalls at +39 position in the absence of UTP in the reaction mix). Transcripts were analyzed by resolving on 20% urea PAGE.

%I PLOS ONE