Vilasi, Silvia Sarcina, Rosalba Maritato, Rosa De Simone, Antonella Irace, Gaetano Sirangelo, Ivana Effect of heparin on W7FW14F apomyoglobin at pH 4.0. <p><b>Panel A</b>: Absorbance at 280 nm of supernatant solution in the absence (▪) and in the presence of 0.1 mg/mL heparin (•). Protein concentration was 40 µM. Points are experimental values, continues line was obtained from an exponential fit as A<sub>280 nm</sub>(t) = A<sub>280 nm</sub>(∞)+A<sub>1</sub> e<sup>−kt</sup>. <b>Panel B</b>: Time-dependence of the far-UV CD spectra in the presence of 0.1 mg/mL heparin. From the lower to the upper spectrum, times are: 20, 40, 60, 120, 180, 300 and 360 min. The protein samples (40 µM) were diluted 1∶2 before spectra acquisition. In red is displayed the spectrum recorded in the absence of heparin. <b>Panel C</b>: ThT fluorescence in the absence (▪) and in the presence of 0.1 mg/mL heparin (•). Protein concentration was 40 µM. Other experimental details are reported under “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0022076#s2" target="_blank">Materials and Methods</a>”.</p> heparin;w7fw14f;apomyoglobin;ph 2013-02-20
    https://plos.figshare.com/articles/figure/_Effect_of_heparin_on_W7FW14F_apomyoglobin_at_pH_4_0_/428172
10.1371/journal.pone.0022076.g005