Nedellec, Rebecca Coetzer, Mia M. Lederman, Michael E. Offord, Robin Hartley, Oliver E. Mosier, Donald Development of resistance to 5P12-RANTES (5P12). <p><b>A.</b> Weekly increases in p24 capsid antigen during four successive rounds of selection: 5P12 1 (blue, weeks 1–15); 5P12 2 (green, weeks 13–25); 5P12 3 (purple, weeks 24–33); and 5P12 4 (brown, weeks 30–44). Control cultures with no inhibitor are shown in black filled circles. <b>B.</b> Increasing concentrations of 5P12 expressed as multiples of the 90% inhibitory concentration (IC<sub>90</sub> = 0.12 nM) for each of the four rounds of selection, with colors matching panel A. <b>C.</b> Replication of viruses from indicated weeks of selection on activated CD4<sup>+</sup> T cells from a CCR5Δ32 homozygous donor. Values are mean p24 capsid antigen levels (± SE of triplicate cultures) after 7 days of culture. <b>D.</b> Viruses from the indicated weeks of 5P12-RANTES round 4 of selection (5P12 4) or control cultures with no inhibitors were used to infect activated CD4<sup>+</sup> T cells from normal donors in the presence of the CXCR4 blocking agent AMD3100 (AMD). The percent inhibition by AMD3100 of p24 capsid antigen levels after 7 days of culture is plotted versus the week of virus isolation.</p> 5p12-rantes 2013-02-20
    https://plos.figshare.com/articles/figure/_Development_of_resistance_to_5P12_RANTES_5P12_/427883
10.1371/journal.pone.0022020.g002