China, Arnab Mishra, Sonakshi Nagaraja, Valakunja (A) Intrinsic transcript cleavage property of RNAP. <p>Stalled elongation complexes bearing the 20 mer transcript were generated with <i>M. smegmatis</i> (<i>Ms</i>), <i>M. tuberculosis</i> (<i>Mtb</i>) and <i>E. coli</i> (<i>Ec</i>) RNAP respectively. The complexes were incubated for a prolonged time (1–4 hrs) in transcription buffer (pH 7.5), followed by resolving the cleavage products on 20% urea-PAGE. <b>(B)</b> pH-induced transcript-cleavage activity of RNAP. The gels show cleaved RNA generated from the 20 mer ternary complexes formed by <i>Ms</i>, <i>Mtb</i> and <i>Ec</i> RNAP in buffers of pH 6.0 to 10.0.</p> intrinsic;transcript;cleavage 2011-07-08
    https://plos.figshare.com/articles/figure/_A_Intrinsic_transcript_cleavage_property_of_RNAP_/427882
10.1371/journal.pone.0021941.g001