Szaszák, Márta Steven, Philipp Shima, Kensuke Orzekowsky-Schröder, Regina Hüttmann, Gereon R. König, Inke Solbach, Werner Rupp, Jan Effects of IFNγ treatment on NAD(P)H inside chlamydial inclusions. <p>HEp-2 cells were infected with <i>C. trachomatis</i> for 24 hours and treated with 10 units /ml IFNγ 24 hours prior to and during the infection (right panels) or were left untreated (left panels). (A) Color-coded images of τ<sub>2</sub>-NAD(P)H (scale bar = 20 µm). Arrows point to persistent chlamydial inclusions harbouring enlarged reticulate bodies. (B) Color-coded images of the relative amount of protein-bound a<sub>2</sub>-NAD(P)H (scale bar = 20 µm). Arrows point to persistent chlamydial inclusions harbouring enlarged reticulate bodies. (C) IFNγ induced persistence decreases τ<sub>2</sub>-NAD(P)H inside the chlamydial inclusion. Quantitative analysis of τ<sub>2</sub>-NAD(P)H (n = 27 ROIs from three independent experiments; mean ±SEM) at 24 hpi. (D) IFNγ induced persistence decreases the sizes of chlamydial inclusions. Quantitative analyzes of chlamydial inclusion sizes at 24 hpi (n = 27 inclusions from three independent experiments; mean ±SEM). (E) IFNγ induced persistence increases the ratio of a<sub>1</sub>/a<sub>2</sub> inside the chlamydial inclusions. Quantitative analysis of ratio of free to protein-bound NAD(P)H (a<sub>1</sub>/a<sub>2</sub>) (n = 27 ROIs from three independent experiments; mean ±SEM).</p> chlamydial 2011-07-14
    https://plos.figshare.com/articles/figure/_Effects_of_IFN_947_treatment_on_NAD_P_H_inside_chlamydial_inclusions_/427881
10.1371/journal.ppat.1002108.g005