10.1371/journal.pone.0167044.g003 Hua Zhao Hua Zhao Kalani Ruberu Kalani Ruberu Hongyun Li Hongyun Li Brett Garner Brett Garner HT1080 cellular uptake of <sup>57</sup>Co-Cbl in the presence of either FCS or HS with or without heat treatment. Public Library of Science 2016 cell culture protocols HT 1080 cells fibrosarcoma-derived fibroblast cell line cell culture conditions HT 1080 cell 57 Co-Cbl uptake Cbl uptake AG 01518 cells cell Type-Specific Modulation cell culture medium calf serum 57 Co-labelled cobalamin Bovine Serum Tracking 57 Co-Cbl uptake TC 2016-11-28 17:38:07 Figure https://plos.figshare.com/articles/figure/HT1080_cellular_uptake_of_sup_57_sup_Co-Cbl_in_the_presence_of_either_FCS_or_HS_with_or_without_heat_treatment_/4266692 <p><b><i>A</i>,</b> FCS was heated at either 95°C for 10 min or 100°C for 20 min before <sup>57</sup>Co-Cbl addition, then incubated with HT1080 cells for 48 h and compared to standard FCS culture conditions. <b><i>B</i>,</b> Using the same 95°C for 10 min FCS heating conditions used in “A”, a time-course study with samples taken at 2 h, 4 h, 8 h, 12 h, 24 h, and 48 h was undertaken. <b><i>C</i></b>, For the purpose of comparison, a time-course study with heated HS (95°C for 10 min) was also conducted in parallel. Data are mean values <u>+</u> SE (n = 3).</p>