Zhou, Yajing Chen, Huiqing Li, Xiao Wang, Yujue Chen, Keping Zhang, Sufang Meng, Xiao Y. C. Lee, Ernest Y. W. T. Lee, Marietta A strategy for assembling multigene expression cassettes in pFBDM. <p>Four genes, p125, p50, p68, and p12, were inserted into MCS1 between <i>Bam</i>HI and <i>Xba</i>I sites, respectively. The expression cassette containing p125 was excised by digestion with <i>Avr</i>II and <i>Pme</i>I (boxed) and placed into a multiplication module (M) of a construct containing p50 via <i>Bst</i>Z17I/<i>Spe</i>I sites. Followed the same logic, a pFBDM-[p125-p50-p68] was generated by an insertion of the expression cassette containing p125 and p50 into a construct containing p68. Finally, a recombinant transfer vector pFBDM-[p125-p50-p68-p12] was generated in which each subunit is under the control of individual <i>polyhedrin</i> promoter.</p> assembling;multigene;cassettes 2011-07-15
    https://plos.figshare.com/articles/figure/_A_strategy_for_assembling_multigene_expression_cassettes_in_pFBDM_/426394
10.1371/journal.pone.0022224.g001