Comparison of circulating miRNAs predicted to target <i>Bmal1</i> or other genes regulating <i>Bmal1</i> expression with a highly abundant miRNA in serum, miR-16.
Vikram R. Shende
Marianna M. Goldrick
Suchitra Ramani
David J. Earnest
10.1371/journal.pone.0022586.g001
https://plos.figshare.com/articles/figure/_Comparison_of_circulating_miRNAs_predicted_to_target_Bmal1_or_other_genes_regulating_Bmal1_expression_with_a_highly_abundant_miRNA_in_serum_miR_16_/423562
<p>(<b>A</b>) Relative expression of miR-16, miR-142-3p, miR-152, miR-494, miR-135b, miR-135a and miR-34c in serum samples collected from mice at ZT 7 (n = 3). Bars denote real-time PCR determinations of serum miRNA levels (mean ± SEM) and the values are plotted using a logarithmic scale in comparison with the average for miR-34c expression. (<b>B</b>) Quantification of miR-16 and miR-152 expression in serum collected from mice at ZT 7 (n = 3). Symbols denote determinations (in duplicate) of the number of copies/µl serum in each sample that were extrapolated by comparing the Ct values for experimental samples with standard curves consisting of a dilution series of known quantities of synthetic miR-16 and miR-152 analyzed on the same plate.</p>
2011-07-22 00:59:22
circulating
mirnas
genes
regulating
abundant
mirna