BDNF promotes cell proliferation, differentiation, and migration of cultured neurospheres.
Wen-Di Lee
Kuo-Chuan Wang
Yi-Fen Tsai
Pin-Chun Chou
Li-Kai Tsai
Chung-Liang Chien
10.1371/journal.pone.0165460.g006
https://plos.figshare.com/articles/figure/BDNF_promotes_cell_proliferation_differentiation_and_migration_of_cultured_neurospheres_/4228868
<p>Neurospheres were immunostained with Ki67, nestin, DCX and GFAP antibodies to determine the effects of recombinant BDNF (5 pg/ml) on neurogenesis of cultured neurospheres. The groups of neurospheres treated with 0.5% CSF (collected from rats on day 7 post SAH) and without treatment (control) were used for comparison. (A)-(I): photomicrograph showing the distribution of Ki67<sup>+</sup> (A, D, and G) and nestin+ (B, E, and H) signals and as merged images (C, F, and I); (J)-(R): photomicrograph showing the distribution of DCX<sup>+</sup> (J, M, and P) and GFAP<sup>+</sup> (K, N, and Q) signals and as merged images (L, O and R). Scale bar = 50 μm. (S): Percentage of proliferating cells (Ki67<sup>+</sup> of Hoechst<sup>+</sup> cells) in the neurospheres. (T): Percentage of DCX<sup>+</sup> area (DCX<sup>+</sup> area/ total area). (U): Percentage of GFAP<sup>+</sup> area (GFAP<sup>+</sup> area/ total area). Means ± SD, 12 neurospheres were counted for each condition. ns: non-significant; ***, P < 0.001, one way ANOVA with Tukey's multiple comparisons test.</p>
2016-11-10 17:53:59
NSC
brain-derived neurotrophic factor
Neural Stem Cells
proliferation
differentiation
7 post SAH
Subarachnoid Hemorrhage Promotes Proliferation
BDNF Upregulation Patients
ELISA
CSF
SVZ
Enzyme-linked immunosorbent assay
days 5
neurogenesis mechanism