Effect of substrate concentrations on excretion rates (a), intracellular amounts (b), CL (c), and <i>f</i><sub>met</sub> (d) of Dio-7-G, Dio-3′-G, Chr-7-G, and Chr-4′-G. Xuejun Zeng Jian Shi Min Zhao Qingwei Chen Liping Wang Huangyu Jiang Feifei Luo Lijun Zhu Linlin Lu Xinchun Wang Zhongqiu Liu 10.1371/journal.pone.0166239.g006 https://plos.figshare.com/articles/figure/Effect_of_substrate_concentrations_on_excretion_rates_a_intracellular_amounts_b_CL_c_and_i_f_i_sub_met_sub_d_of_Dio-7-G_Dio-3_-G_Chr-7-G_and_Chr-4_-G_/4226498 <p>Three samples (500 μL) were obtained at 15, 30, and 60 min and replaced with fresh loading solution (500 μL) that contains diosmetin or chrysoeriol. The excretion rates of glucuronides were calculated from the slope of the amount-versus-time curves. The intracellular amounts of the glucuronides were determined at the end of the excretion experiments after the cells were washed twice with ice-cold HBSS. Each column corresponds to the average of three determinations with error bars representing the S.D. The “*” (for Dio-7-G or Chr-7-G) or “<sup>#</sup>” (for Dio-3′-G or Chr-4′-G) symbol means a statistically significant difference between-group, at p < 0.05; “**” or “<sup>##</sup>” means p < 0.01; “***” or “<sup>###</sup>” means p < 0.001.</p> 2016-11-10 17:34:20 diosmetin UGT 1A HeLa Cells chrysoeriol glucuronides OH UGT 1A UGT 1A BCRP HeLa cells overexpressing UGT 1A breast cancer resistance protein HLM regioselective glucuronidation UGT 1A Importantly