Effect of substrate concentrations on excretion rates (a), intracellular amounts (b), CL (c), and <i>f</i><sub>met</sub> (d) of Dio-7-G, Dio-3′-G, Chr-7-G, and Chr-4′-G.
Xuejun Zeng
Jian Shi
Min Zhao
Qingwei Chen
Liping Wang
Huangyu Jiang
Feifei Luo
Lijun Zhu
Linlin Lu
Xinchun Wang
Zhongqiu Liu
10.1371/journal.pone.0166239.g006
https://plos.figshare.com/articles/figure/Effect_of_substrate_concentrations_on_excretion_rates_a_intracellular_amounts_b_CL_c_and_i_f_i_sub_met_sub_d_of_Dio-7-G_Dio-3_-G_Chr-7-G_and_Chr-4_-G_/4226498
<p>Three samples (500 μL) were obtained at 15, 30, and 60 min and replaced with fresh loading solution (500 μL) that contains diosmetin or chrysoeriol. The excretion rates of glucuronides were calculated from the slope of the amount-versus-time curves. The intracellular amounts of the glucuronides were determined at the end of the excretion experiments after the cells were washed twice with ice-cold HBSS. Each column corresponds to the average of three determinations with error bars representing the S.D. The “*” (for Dio-7-G or Chr-7-G) or “<sup>#</sup>” (for Dio-3′-G or Chr-4′-G) symbol means a statistically significant difference between-group, at p < 0.05; “**” or “<sup>##</sup>” means p < 0.01; “***” or “<sup>###</sup>” means p < 0.001.</p>
2016-11-10 17:34:20
diosmetin
UGT 1A HeLa Cells
chrysoeriol glucuronides
OH
UGT 1A UGT 1A
BCRP
HeLa cells overexpressing UGT 1A
breast cancer resistance protein
HLM
regioselective glucuronidation
UGT 1A Importantly