10.1371/journal.pone.0021721.g001 Hitoshi Yokoyama Hitoshi Yokoyama Tamae Maruoka Tamae Maruoka Haruki Ochi Haruki Ochi Akio Aruga Akio Aruga Shiro Ohgo Shiro Ohgo Hajime Ogino Hajime Ogino Koji Tamura Koji Tamura <i>Wnt-3a</i> is expressed in the blastema of both froglets and tadpoles. Public Library of Science 2011 blastema froglets 2011-07-26 00:43:29 Figure https://plos.figshare.com/articles/figure/_Wnt_3a_is_expressed_in_the_blastema_of_both_froglets_and_tadpoles_/422609 <p>(A) RT-PCR of the total RNA from tadpole blastemas at 5 dpa or froglet blastemas at 9 dpa showed that_<i>wnt-3a</i> was expressed in both the tadpole and froglet blastemas. (B) In situ hybridization of froglet blastemas at 9 dpa with a <i>wnt-3a</i> antisense probe revealed expression of <i>wnt-3a</i> in the epidermal layer of the blastema, while no specific signal was detected with the <i>wnt-3a</i> sense probe. Each section was hybridized with the antisense or sense probe, at the same time and by the same procedure, and the development of the staining reaction was stopped at exactly the same time. The lines indicate the estimated amputation plane. Scale bar = 100 µm.</p>