10.1371/journal.pone.0021721.g001
Hitoshi Yokoyama
Hitoshi
Yokoyama
Tamae Maruoka
Tamae
Maruoka
Haruki Ochi
Haruki
Ochi
Akio Aruga
Akio
Aruga
Shiro Ohgo
Shiro
Ohgo
Hajime Ogino
Hajime
Ogino
Koji Tamura
Koji
Tamura
<i>Wnt-3a</i> is expressed in the blastema of both froglets and tadpoles.
Public Library of Science
2011
blastema
froglets
2011-07-26 00:43:29
Figure
https://plos.figshare.com/articles/figure/_Wnt_3a_is_expressed_in_the_blastema_of_both_froglets_and_tadpoles_/422609
<p>(A) RT-PCR of the total RNA from tadpole blastemas at 5 dpa or froglet blastemas at 9 dpa showed that_<i>wnt-3a</i> was expressed in both the tadpole and froglet blastemas. (B) In situ hybridization of froglet blastemas at 9 dpa with a <i>wnt-3a</i> antisense probe revealed expression of <i>wnt-3a</i> in the epidermal layer of the blastema, while no specific signal was detected with the <i>wnt-3a</i> sense probe. Each section was hybridized with the antisense or sense probe, at the same time and by the same procedure, and the development of the staining reaction was stopped at exactly the same time. The lines indicate the estimated amputation plane. Scale bar = 100 µm.</p>