Norgestrel-supplemented diet significantly protects against photoreceptor cell death out to P40 in the rd10 retina. Sarah L. Roche Alice C. Wyse-Jackson Violeta Gómez-Vicente Pedro Lax Ana M. Ruiz-Lopez Ashleigh M. Byrne Nicolás Cuenca Thomas G. Cotter 10.1371/journal.pone.0165197.g001 https://plos.figshare.com/articles/figure/Norgestrel-supplemented_diet_significantly_protects_against_photoreceptor_cell_death_out_to_P40_in_the_rd10_retina_/4211085 <p><b>(A)</b> Apoptosis of retinal cells was detected by terminal dUTP nick end-labeling (TUNEL; green & greyscale) of DNA strand breaks in cell nuclei. Norgestrel treatment (NORG diet) decreased TUNEL-positive staining in the central P15 and P20 retina compared to control mice (Control diet). N = 3 mice per group. Hoechst (blue) staining reveals the cell layers in the retina. Scale bar 50μm. <b>(B)</b> Quantification of outer nuclear layer (ONL) thickness in the central and peripheral retina. N = 3 mice per group, n = 20 sections per mouse. Results are presented as mean ± SEM (t-test, *p<0.05, **p<0.01, ***p<0.005).</p> 2016-11-04 17:30:02 RP neuroprotective mouse models 661 W photoreceptor-like cells photoreceptor cell loss rd 10 microglia drive Protective Fractalkine-CX 3CR Signaling Retinitis pigmentosa upregulating growth factors DAMP RNA rd 10 mouse 10 rd 10 pups Norgestrel upregulates fractalkine-CX 3CR Progesterone Attenuates Microglial-Driven Retinal Degeneration implicating microglial changes rd 10 retina P 15-P rd 10 mice rd 10 mice cell death