10.1371/journal.pgen.1006412.g002 Haijiao Dong Haijiao Dong Hu Zhao Hu Zhao Weibo Xie Weibo Xie Zhongmin Han Zhongmin Han Guangwei Li Guangwei Li Wen Yao Wen Yao Xufeng Bai Xufeng Bai Yong Hu Yong Hu Zilong Guo Zilong Guo Kai Lu Kai Lu Lin Yang Lin Yang Yongzhong Xing Yongzhong Xing Mutants of the candidate gene for <i>qTA3</i> showing a larger tiller angle. Public Library of Science 2016 TAC 1 TAC 3 Tiller Angle Control 3 Novel Tiller Angle Gene GWAS novel gene TAC 3 genome-wide association studies tiller angle D 2 indica qTA 3 QTL 156 japonica accessions DWARF nucleotide diversity analysis controls tiller angle gene Tiller Angle Control 1 Rice Cultivars Tiller angle 2016-11-04 17:30:02 Figure https://plos.figshare.com/articles/figure/Mutants_of_the_candidate_gene_for_i_qTA3_i_showing_a_larger_tiller_angle_/4211043 <p>(a) A representation of pairwise <i>r<sup>2</sup></i> values (a measure of LD) all polymorphic sites between <i>Os03g51660</i> and <i>Os03g51670</i>, where the darkness of the color of each box corresponds to the <i>r<sup>2</sup></i> value according to the legend. The line in red color represents lead SNP. (b) Schematic diagram of <i>Os03g51660</i> and <i>Os03g51670</i> structures with the T-DNA insertion. The black squares represent exons, and the black lines represent introns. The red line means the position of the lead SNP of <i>qTA3</i>; the triangles indicate the positions of T-DNA insertion in three mutants; L and R, left and right genomic primers; N, vector primer. (c) (d)Genotyping of the mutants <i>tac3-1D</i> and <i>tac3-2D</i>. W, wild type; H, heterozygote; M, homozygote. (e) qRT-PCR expression analysis of <i>Os03g51660</i> in wild type (ZH11) and <i>tac3-1D</i> mutant using leaves on the beginning of tillering stage; the number of plants in each genotype (n) ≥ 9, *** significant difference between mutants and wild type at p<0.001. (f) qRT-PCR expression analysis of <i>Os03g51670</i> in ZH11 and <i>tac3-1D</i> mutant using leaves on the beginning of tillering stage; the number of plants in each genotype (n) ≥4 plants, *** significant difference between mutants and wild type at p<0.001. (g)-(i) The tiller angle of ZH11, heterozygote (<i>tac3-1D</i> H) and homozygote (<i>tac3-1D</i> M) mutant at tillering and heading stages; ** and *** significant difference between mutants and wild type at p<0.01 and p<0.001, respectively. (j) qRT-PCR expression analysis of <i>Os03g51660</i> in wild type (DJ) and <i>tac3-2D</i> mutant using leaves on the beginning of tillering stage; the number of plants in each genotype (n) ≥4 plants, *** significant difference between mutants and wild type at p<0.001. (k) qRT-PCR expression analysis of <i>Os03g51670</i> in DJ and <i>tac3-2D</i> mutant using leaves on the beginning of tillering stage; the number of plants in each genotype (n) ≥4 plants. (l)-(n) The tiller angle of DJ, heterozygote (<i>tac3-2D</i> H) and homozygote (<i>tac3-2D</i> M) mutant at tillering and heading stages; the total plant number (n) = 62 plants, ** significant difference between mutants and wild type at p<0.01.</p>