10.1371/journal.pgen.1006412.g002
Haijiao Dong
Haijiao
Dong
Hu Zhao
Hu
Zhao
Weibo Xie
Weibo
Xie
Zhongmin Han
Zhongmin
Han
Guangwei Li
Guangwei
Li
Wen Yao
Wen
Yao
Xufeng Bai
Xufeng
Bai
Yong Hu
Yong
Hu
Zilong Guo
Zilong
Guo
Kai Lu
Kai
Lu
Lin Yang
Lin
Yang
Yongzhong Xing
Yongzhong
Xing
Mutants of the candidate gene for <i>qTA3</i> showing a larger tiller angle.
Public Library of Science
2016
TAC 1
TAC 3
Tiller Angle Control 3
Novel Tiller Angle Gene
GWAS
novel gene TAC 3
genome-wide association studies
tiller angle
D 2
indica
qTA 3
QTL
156 japonica accessions
DWARF
nucleotide diversity analysis
controls tiller angle
gene Tiller Angle Control 1
Rice Cultivars Tiller angle
2016-11-04 17:30:02
Figure
https://plos.figshare.com/articles/figure/Mutants_of_the_candidate_gene_for_i_qTA3_i_showing_a_larger_tiller_angle_/4211043
<p>(a) A representation of pairwise <i>r<sup>2</sup></i> values (a measure of LD) all polymorphic sites between <i>Os03g51660</i> and <i>Os03g51670</i>, where the darkness of the color of each box corresponds to the <i>r<sup>2</sup></i> value according to the legend. The line in red color represents lead SNP. (b) Schematic diagram of <i>Os03g51660</i> and <i>Os03g51670</i> structures with the T-DNA insertion. The black squares represent exons, and the black lines represent introns. The red line means the position of the lead SNP of <i>qTA3</i>; the triangles indicate the positions of T-DNA insertion in three mutants; L and R, left and right genomic primers; N, vector primer. (c) (d)Genotyping of the mutants <i>tac3-1D</i> and <i>tac3-2D</i>. W, wild type; H, heterozygote; M, homozygote. (e) qRT-PCR expression analysis of <i>Os03g51660</i> in wild type (ZH11) and <i>tac3-1D</i> mutant using leaves on the beginning of tillering stage; the number of plants in each genotype (n) ≥ 9, *** significant difference between mutants and wild type at p<0.001. (f) qRT-PCR expression analysis of <i>Os03g51670</i> in ZH11 and <i>tac3-1D</i> mutant using leaves on the beginning of tillering stage; the number of plants in each genotype (n) ≥4 plants, *** significant difference between mutants and wild type at p<0.001. (g)-(i) The tiller angle of ZH11, heterozygote (<i>tac3-1D</i> H) and homozygote (<i>tac3-1D</i> M) mutant at tillering and heading stages; ** and *** significant difference between mutants and wild type at p<0.01 and p<0.001, respectively. (j) qRT-PCR expression analysis of <i>Os03g51660</i> in wild type (DJ) and <i>tac3-2D</i> mutant using leaves on the beginning of tillering stage; the number of plants in each genotype (n) ≥4 plants, *** significant difference between mutants and wild type at p<0.001. (k) qRT-PCR expression analysis of <i>Os03g51670</i> in DJ and <i>tac3-2D</i> mutant using leaves on the beginning of tillering stage; the number of plants in each genotype (n) ≥4 plants. (l)-(n) The tiller angle of DJ, heterozygote (<i>tac3-2D</i> H) and homozygote (<i>tac3-2D</i> M) mutant at tillering and heading stages; the total plant number (n) = 62 plants, ** significant difference between mutants and wild type at p<0.01.</p>