Effect of apical plasma membrane LLC-PK1 exosomes with GAPDH depleted activity on ENaC activity in Xenopus 2F3 cells.
Kishore Kumar Jella
Ling Yu
Qiang Yue
Daniel Friedman
Billie J. Duke
Abdel A. Alli
10.1371/journal.pone.0165763.g011
https://plos.figshare.com/articles/figure/Effect_of_apical_plasma_membrane_LLC-PK1_exosomes_with_GAPDH_depleted_activity_on_ENaC_activity_in_Xenopus_2F3_cells_/4196979
<p>(A) GAPDH activity was measured after lysing apical plasma membrane LLC-PK1 exosomes treated with the potent and selective GAPDH inhibitor Heptelidic acid (Koningic acid). (B) Representative single-channel patch clamp recording from the control and treated group. (C-E) Summary of all single channel data. The summary bar graph in (C) shows ENaC activity, NPo. The summary bar graph in (D) shows the number of channels, N. The summary bar graph in (E) shows the open probability, Po for the control and treated groups. Data is presented as the mean ± s.e. and the number of patches is indicated above each group.</p>
2016-11-01 17:37:24
Xenopus 2 F 3 cells
tubule cells
tubule Xenopus 2 F 3 cells
LLC-PK 1 cells
GAPDH inhibitor heptelidic acid
lipophilic dye PKH 26
Proximal Tubule Cells Regulate ENaC Activity Exosomes
CTX
tubule LLC-PK 1 cells
epithelial sodium channels
exosome
mpkCCD cells
ENaC activity
Cultured mpkCCD cells