10.1371/journal.pone.0024082.g003 Shreeram C. Nallar Shreeram C. Nallar Limei Lin Limei Lin Varsha Srivastava Varsha Srivastava Padmaja Gade Padmaja Gade Edward R. Hofmann Edward R. Hofmann Hafiz Ahmed Hafiz Ahmed Sekhar P. Reddy Sekhar P. Reddy Dhananjaya V. Kalvakolanu Dhananjaya V. Kalvakolanu GRIM-1 regulates small non-coding RNA levels. Public Library of Science 2011 regulates non-coding rna 2011-09-08 02:25:14 Figure https://plos.figshare.com/articles/figure/_GRIM_1_regulates_small_non_coding_RNA_levels_/408714 <p>Relative abundance was calculated with respect to spliceosomal RNA (snRNA-U6) levels by real-time PCR. A) Levels of the indicated RNA in HeLa cells stably expressing Myc-tagged GRIM-1 isoforms. GRIM-1 isoforms differentially suppress box H/ACA-containing RNA. Top: snoRNA-73 (open), -74 (hatched), -50 (light gray); Middle: scaRNA-8 (stippled), -13 (brick), TERC (black); Bottom: spliceosomal RNA (snRNA-U2 (wavy)) and box C/D-containing RNA (snoRNA-U3 (checkered)) levels. A related box C/D snoRNA-U13 (dark gray) levels are not influenced by GRIM-1. B) Levels of the indicated RNA upon GRIM-1 depletion in HeLa cells. GRIM-1-depleted cells (GS) show increased box H/ACA-containing RNA. Top: snoRNA-73, -74 and -50; Middle: scaRNA-8, -13, TERC; Bottom: snRNA-U2 and snoRNA-U3 levels. A related box C/D snoRNA-U13 levels is not influenced by GRIM-1. Bar patterns same as in (A). **, <i>p</i><0.01.</p>