10.1371/journal.pone.0024568.g001
Monika Kwiecinski
Monika
Kwiecinski
Andrea Noetel
Andrea
Noetel
Natalia Elfimova
Natalia
Elfimova
Jonel Trebicka
Jonel
Trebicka
Stephanie Schievenbusch
Stephanie
Schievenbusch
Ingo Strack
Ingo
Strack
Levente Molnar
Levente
Molnar
Melanie von Brandenstein
Melanie
von Brandenstein
Ulrich Töx
Ulrich
Töx
Roswitha Nischt
Roswitha
Nischt
Oliver Coutelle
Oliver
Coutelle
Hans Peter Dienes
Hans
Peter Dienes
Margarete Odenthal
Margarete
Odenthal
Met and HGF expression in primary and immortalized HSC.
Public Library of Science
2011
hgf
immortalized
2011-09-09 01:57:48
Figure
https://plos.figshare.com/articles/figure/_Met_and_HGF_expression_in_primary_and_immortalized_HSC_/407068
<p>Total RNA was extracted from primary rat HSC after the third, fifth and seventh day of cell culture (3d, 5d, 7d) and from myofibroblastic HSC-T6 cells. Subsequently, c-met and HGF expression was determined (A–C). The c-met and HGF transcript levels were quantified by Real-Time PCR and normalized using HPRT as house-keeping gene (A–C). The expression of c-met increased during the differentiation process of primary HSC and achieved the highest level in the immortalized cell line HSC-T6 representing a myofibroblastic phenotype <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0024568#pone.0024568-Vogel1" target="_blank">[41]</a>, whereas HGF levels were shown to be opposite (A, B). Primary HSC at the 3rd culture day (prim. HSC) and immortalized HSC (HSC-T6) were either untreated (−) or stimulated with TGF-β (+) and RNA levels of c-met and HGF were analyzed by Real-Time PCR (C). The Met protein expression in HGF and TGF-β stimulated HSC-T6 (+HGF, +TGF-β) in comparison to untreated HSC-T6 cells (control) was shown by Western blot analysis (D).</p>