10.1371/journal.pone.0024568.g001 Monika Kwiecinski Monika Kwiecinski Andrea Noetel Andrea Noetel Natalia Elfimova Natalia Elfimova Jonel Trebicka Jonel Trebicka Stephanie Schievenbusch Stephanie Schievenbusch Ingo Strack Ingo Strack Levente Molnar Levente Molnar Melanie von Brandenstein Melanie von Brandenstein Ulrich Töx Ulrich Töx Roswitha Nischt Roswitha Nischt Oliver Coutelle Oliver Coutelle Hans Peter Dienes Hans Peter Dienes Margarete Odenthal Margarete Odenthal Met and HGF expression in primary and immortalized HSC. Public Library of Science 2011 hgf immortalized 2011-09-09 01:57:48 Figure https://plos.figshare.com/articles/figure/_Met_and_HGF_expression_in_primary_and_immortalized_HSC_/407068 <p>Total RNA was extracted from primary rat HSC after the third, fifth and seventh day of cell culture (3d, 5d, 7d) and from myofibroblastic HSC-T6 cells. Subsequently, c-met and HGF expression was determined (A–C). The c-met and HGF transcript levels were quantified by Real-Time PCR and normalized using HPRT as house-keeping gene (A–C). The expression of c-met increased during the differentiation process of primary HSC and achieved the highest level in the immortalized cell line HSC-T6 representing a myofibroblastic phenotype <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0024568#pone.0024568-Vogel1" target="_blank">[41]</a>, whereas HGF levels were shown to be opposite (A, B). Primary HSC at the 3rd culture day (prim. HSC) and immortalized HSC (HSC-T6) were either untreated (−) or stimulated with TGF-β (+) and RNA levels of c-met and HGF were analyzed by Real-Time PCR (C). The Met protein expression in HGF and TGF-β stimulated HSC-T6 (+HGF, +TGF-β) in comparison to untreated HSC-T6 cells (control) was shown by Western blot analysis (D).</p>