BMASCT restores the ISEMF and pericryptal macrophages of the ISC niche, 3.5 days post-AIR. Subhrajit Saha Payel Bhanja Rafi Kabarriti Laibin Liu Alan A. Alfieri Chandan Guha 10.1371/journal.pone.0024072.g005 https://plos.figshare.com/articles/figure/_BMASCT_restores_the_ISEMF_and_pericryptal_macrophages_of_the_ISC_niche_3_5_days_post_AIR_/405066 <p><b>A. </b><u>ISEMF detection by immunohistochemistry and confocal microscopy using anti-α-SMA (stained red, indicated with arrow) and anti-desmin (stained green) antibodies</u>. α-SMA+ve and desmin−ve ISEMF were reduced in AIR-treated animals, which was restored by BMASCT. Nucleus was stained with DAPI (blue). <b>B<u>.</u></b><u> F480 Immunhistochemistry and confocal microscopic analysis</u> and <b>C.</b><u> Quantification of Number of pericryptal macrophages</u>. The number of F480+ve macrophages (green, indicated with arrow) increased at 3.5 d post-AIR in the AIR+BMASCT (p<0.003) and CD11b+ve BMASCT (p<0.006) group, compared to the AIR cohort, respectively. Nucleus was stained with DAPI (pseudo colored with red). Confocal microscopic images (63×) were magnified 2.3× (inset).</p> 2011-09-15 01:24:26 restores isemf pericryptal macrophages isc days