10.1371/journal.pone.0024250.g003 Lauren A. Hirao Lauren A. Hirao David A. Hokey David A. Hokey Matthew P. Morrow Matthew P. Morrow Maria N. Jure-Kunkel Maria N. Jure-Kunkel David B. Weiner David B. Weiner Polyfunctional profile of CD4<sup>+</sup> T cells. Public Library of Science 2013 immunology Infectious diseases 2013-02-20 14:41:55 Figure https://plos.figshare.com/articles/figure/_Polyfunctional_profile_of_CD4_T_cells_/405060 <p>PBMCs isolated 2 weeks after the fourth immunization were stimulated <i>in vitro</i> with a SIVpol peptide pool mix for 5 hours. Cells were stained for intracellular production of IFN-γ, TNF-α and IL-2 and degranulation by CD107a. The magnitude of the SIVgag (white), env (grey) and pol (black) responses are shown as stacked means ± SEM for each group (<b>a</b>). The percentage of the total functional response that has a CD28<sup>−</sup>CD95<sup>+</sup> (black bar) or CD28<sup>+</sup>CD95<sup>+</sup> (white bar) is shown as group means ± SEM (<b>b</b>). Pie charts show the proportion of antigen-specific CD4<sup>+</sup> T cells that have 4 functions (purple), 3 functions (yellow), 2 functions (green) or 1 function (light blue) (<b>c</b>). The bar graphs depict the absolute frequency of each of the 15 functional combinations for the DNA (red), 4-1BB (blue), CTLA-4 (orange), Combo (green) and Saline (black) groups in response to SIVgag, env, and pol after background subtraction (<b>d</b>).</p>