Fan, Xiaolian Tkachyova, Ilona Sinha, Ankit Rigat, Brigitte Mahuran, Don Immobilized [3H]acetylated N-acetyltransferase-His8Flag intermediate cannot be detected in immunoprecipitation experiments with anti-Flag M2 beads. 1<p>Anti-Flag beads were incubated with extracts from HeLa cell expressing (Transfected) or not expressing (Untransfected) N-acetyltransferase-His8Flag, washed and then incubated with [3H]acetyl-CoA for the given time and pH, at room temperature and with or without 1.3 mM of lipids containing 20% PI.</p>2<p>N-acetyltransferase activity in nmoles of MU produced/hour, and the concentration of protein (pmoles), calculated based on the specific activity of the purified transferase, are given.</p>3<p>The data represent three independent beads binding experiments and assays.</p>4<p>Not detectable.</p> n-acetyltransferase-his8flag;intermediate;cannot;immunoprecipitation;experiments;anti-flag;m2 2013-02-20
    https://plos.figshare.com/articles/dataset/_Immobilized_3H_acetylated_N_acetyltransferase_His8Flag_intermediate_cannot_be_detected_in_immunoprecipitation_experiments_with_anti_Flag_M2_beads_/404686
10.1371/journal.pone.0024951.t002