%0 Generic %A Fan, Xiaolian %A Tkachyova, Ilona %A Sinha, Ankit %A Rigat, Brigitte %A Mahuran, Don %D 2013 %T Immobilized [3H]acetylated N-acetyltransferase-His8Flag intermediate cannot be detected in immunoprecipitation experiments with anti-Flag M2 beads. %U https://plos.figshare.com/articles/dataset/_Immobilized_3H_acetylated_N_acetyltransferase_His8Flag_intermediate_cannot_be_detected_in_immunoprecipitation_experiments_with_anti_Flag_M2_beads_/404686 %R 10.1371/journal.pone.0024951.t002 %2 https://plos.figshare.com/ndownloader/files/734337 %K n-acetyltransferase-his8flag %K intermediate %K cannot %K immunoprecipitation %K experiments %K anti-flag %K m2 %X 1

Anti-Flag beads were incubated with extracts from HeLa cell expressing (Transfected) or not expressing (Untransfected) N-acetyltransferase-His8Flag, washed and then incubated with [3H]acetyl-CoA for the given time and pH, at room temperature and with or without 1.3 mM of lipids containing 20% PI.

2

N-acetyltransferase activity in nmoles of MU produced/hour, and the concentration of protein (pmoles), calculated based on the specific activity of the purified transferase, are given.

3

The data represent three independent beads binding experiments and assays.

4

Not detectable.

%I PLOS ONE