10.1371/journal.pone.0025222.g005 Jelena Põlajeva Jelena Põlajeva Anna M. Sjösten Anna M. Sjösten Nina Lager Nina Lager Marianne Kastemar Marianne Kastemar Ida Waern Ida Waern Irina Alafuzoff Irina Alafuzoff Anja Smits Anja Smits Bengt Westermark Bengt Westermark Gunnar Pejler Gunnar Pejler Lene Uhrbom Lene Uhrbom Elena Tchougounova Elena Tchougounova Co-expression of CXCR4 and mMCP-6 by MCs in mouse RCAS-<i>KRas</i>+RCAS-<i>Akt</i> induced gliomas. Public Library of Science 2013 cxcr4 mmcp-6 mcs induced 2013-02-20 14:39:50 Figure https://plos.figshare.com/articles/figure/_Co_expression_of_CXCR4_and_mMCP_6_by_MCs_in_mouse_RCAS_KRas_RCAS_Akt_induced_gliomas_/404685 <p>(<b>A</b>) Immunofluorescence staining for CXCR4 and mMCP-6 was performed in both <i>Ntv-a Arf−/−</i> and <i>Gtv-a Arf−/−</i> mouse gliomas, demonstrating co-expression of CXCR4 and mMCP-6. Scale bar = 50 µM. (<b>B</b>) Immunofluorescence staining, demonstrated co-localization of CXCR4 and mMCP-6 at the single-cell level in both <i>Ntv-a Arf−/−</i> and <i>Gtv-a Arf−/−</i> mouse gliomas. Maximum intensity projection of z-stack confocal images was applied. Scale bar = 20 µM. (<b>C</b>) The quantification of MCs in mouse brain tumors revealed about 90% to be CXCR4-positive in the corresponding objective fields with no difference between <i>Ntv-a Arf−/−</i> (n = 5) and <i>Gtv-a Arf−/−</i> (n = 5). Error bars show SD.</p>