Validation of the RNA-Seq results using quantitative real-time PCR. HuangJian ZhaoLei YangPing ChenZhen TangNi Z. RuanXiong ChenYaxi 2016 <p>The values are presented as the means±SD (n = 4). Comparisons among groups were determined with independent-sample t-test using the SPSS software (Version 11.5). Significant differences are considered at *P<0.05, ** p < 0.001 and *** p < 0.0001 versus control group.β-actin was used as the reference gene.</p>