10.1371/journal.pone.0164693.g004 Shiho Sugawara Shiho Sugawara Masahiko Maeno Masahiko Maeno Cliff Lee Cliff Lee Shigemi Nagai Shigemi Nagai David M. Kim David M. Kim John Da Silva John Da Silva Masazumi Nagai Masazumi Nagai Hisatomo Kondo Hisatomo Kondo Adhesion of OBA9 epithelial cells to PRP pre-incubated titanium surfaces. Public Library of Science 2016 spread laminin 5 EGF VEGF epithelial sealing Titanium Surface Coated Platelet Activating Peptide platelet aggregates IGF-I titanium surface implant abutment surface epithelial BM-attachment Human gingival epithelial cells TGF PAR 4-AP PAR 4-AP coating ZO 2016-10-14 17:32:33 Figure https://plos.figshare.com/articles/figure/Adhesion_of_OBA9_epithelial_cells_to_PRP_pre-incubated_titanium_surfaces_/4034229 <p>Adhered OBA9 cells were counted by nuclear DNA staining with Hoechst 33342. Each data point represents mean ± SD of three independent cultures. Kinetic adhesion was determined in the titanium that was pre-incubated with PRP from one representative donor (a). The adhesion assay was triplicated at 60 minutes with PRP from different donors (b). ** indicates a significant difference than control at the corresponding time point and in the corresponding donors (**<i>p < 0</i>.<i>01</i>). Supplemental data is published in <a href="http://dx.doi.org/10.7910/DVN/FDMPZY" target="_blank">http://dx.doi.org/10.7910/DVN/FDMPZY</a>.</p>