Southern blot hybridization results.
Yanzheng Song
Fengju Zhang
Yanyan Zhao
Mingshen Sun
Jun Tao
Yanchuang Liang
Ling Ma
Yanqiu Yu
Jianhua Wang
Junfeng Hao
10.1371/journal.pone.0163165.g002
https://plos.figshare.com/articles/figure/Southern_blot_hybridization_results_/3998667
<p>The genomic DNA extracted from six transgenic mice and one wild-type mouse was digested with EcoR I and Bgl II and hybridized using a DIGD-110-labeled probe. Lane 1 to lane 6, EcoR I- and Bgl II-digested DNA from six mouse tails (No. 9, No. 19, No. 20, No. 22, No. 24, and No. 31). Lane 7, genomic DNA fragments from the wild-type mouse as a negative control. Lane 8 to lane 9, PCR products of transgene <i>LUM</i> as positive controls.</p>
2016-10-06 17:39:51
Altered Ultrastructural Features
wild-type B 6 mice
sclera
collagen type
Western blot analysis
COL
ultrastructural
collagen fibrils
collagen fibril diameter
MYP 3 locus
length
Mutant Lumican Transgenic Mouse Model Lumican
LUM
gene expression levels
TEM
polymerase chain reaction
transmission electron microscopy
myopia
mouse model
L 199P
protein levels
lumican