Southern blot hybridization results. Yanzheng Song Fengju Zhang Yanyan Zhao Mingshen Sun Jun Tao Yanchuang Liang Ling Ma Yanqiu Yu Jianhua Wang Junfeng Hao 10.1371/journal.pone.0163165.g002 https://plos.figshare.com/articles/figure/Southern_blot_hybridization_results_/3998667 <p>The genomic DNA extracted from six transgenic mice and one wild-type mouse was digested with EcoR I and Bgl II and hybridized using a DIGD-110-labeled probe. Lane 1 to lane 6, EcoR I- and Bgl II-digested DNA from six mouse tails (No. 9, No. 19, No. 20, No. 22, No. 24, and No. 31). Lane 7, genomic DNA fragments from the wild-type mouse as a negative control. Lane 8 to lane 9, PCR products of transgene <i>LUM</i> as positive controls.</p> 2016-10-06 17:39:51 Altered Ultrastructural Features wild-type B 6 mice sclera collagen type Western blot analysis COL ultrastructural collagen fibrils collagen fibril diameter MYP 3 locus length Mutant Lumican Transgenic Mouse Model Lumican LUM gene expression levels TEM polymerase chain reaction transmission electron microscopy myopia mouse model L 199P protein levels lumican