10.1371/journal.pone.0163165.g002
Yanzheng Song
Yanzheng
Song
Fengju Zhang
Fengju
Zhang
Yanyan Zhao
Yanyan
Zhao
Mingshen Sun
Mingshen
Sun
Jun Tao
Jun
Tao
Yanchuang Liang
Yanchuang
Liang
Ling Ma
Ling
Ma
Yanqiu Yu
Yanqiu
Yu
Jianhua Wang
Jianhua
Wang
Junfeng Hao
Junfeng
Hao
Southern blot hybridization results.
Public Library of Science
2016
Altered Ultrastructural Features
wild-type B 6 mice
sclera
collagen type
Western blot analysis
COL
ultrastructural
collagen fibrils
collagen fibril diameter
MYP 3 locus
length
Mutant Lumican Transgenic Mouse Model Lumican
LUM
gene expression levels
TEM
polymerase chain reaction
transmission electron microscopy
myopia
mouse model
L 199P
protein levels
lumican
2016-10-06 17:39:51
Figure
https://plos.figshare.com/articles/figure/Southern_blot_hybridization_results_/3998667
<p>The genomic DNA extracted from six transgenic mice and one wild-type mouse was digested with EcoR I and Bgl II and hybridized using a DIGD-110-labeled probe. Lane 1 to lane 6, EcoR I- and Bgl II-digested DNA from six mouse tails (No. 9, No. 19, No. 20, No. 22, No. 24, and No. 31). Lane 7, genomic DNA fragments from the wild-type mouse as a negative control. Lane 8 to lane 9, PCR products of transgene <i>LUM</i> as positive controls.</p>