10.1371/journal.pone.0163165.g002 Yanzheng Song Yanzheng Song Fengju Zhang Fengju Zhang Yanyan Zhao Yanyan Zhao Mingshen Sun Mingshen Sun Jun Tao Jun Tao Yanchuang Liang Yanchuang Liang Ling Ma Ling Ma Yanqiu Yu Yanqiu Yu Jianhua Wang Jianhua Wang Junfeng Hao Junfeng Hao Southern blot hybridization results. Public Library of Science 2016 Altered Ultrastructural Features wild-type B 6 mice sclera collagen type Western blot analysis COL ultrastructural collagen fibrils collagen fibril diameter MYP 3 locus length Mutant Lumican Transgenic Mouse Model Lumican LUM gene expression levels TEM polymerase chain reaction transmission electron microscopy myopia mouse model L 199P protein levels lumican 2016-10-06 17:39:51 Figure https://plos.figshare.com/articles/figure/Southern_blot_hybridization_results_/3998667 <p>The genomic DNA extracted from six transgenic mice and one wild-type mouse was digested with EcoR I and Bgl II and hybridized using a DIGD-110-labeled probe. Lane 1 to lane 6, EcoR I- and Bgl II-digested DNA from six mouse tails (No. 9, No. 19, No. 20, No. 22, No. 24, and No. 31). Lane 7, genomic DNA fragments from the wild-type mouse as a negative control. Lane 8 to lane 9, PCR products of transgene <i>LUM</i> as positive controls.</p>